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Academic Journal

Highly selective split intein method for efficient separation and purification of recombinant therapeutic proteins from mammalian cell culture fluid.

  • Authors : Prabhala SV; William G. Lowrie Department of Chemical and Biomolecular Engineering, Ohio State University, Columbus, OH 43210, United States.; Marshall B

Subjects: Recombinant Proteins*/Recombinant Proteins*/Recombinant Proteins*/isolation & purification ; Recombinant Proteins*/Recombinant Proteins*/Recombinant Proteins*/chemistry ; Chromatography, Affinity*/Chromatography, Affinity*/Chromatography, Affinity*/methods

  • Source: Journal of chromatography. A [J Chromatogr A] 2024 Nov 08; Vol. 1736, pp. 465430. Date of Electronic Publication: 2024 Oct 09.Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9318488 Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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Academic Journal

Intein-based Design Expands Diversity of Selenocysteine Reporters.

  • Authors : Chung CZ; Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06511, USA. Electronic address: .; Krahn N

Subjects: Genes, Reporter* ; Inteins*/Inteins*/Inteins*/genetics ; Mutagenesis, Site-Directed*/Mutagenesis, Site-Directed*/Mutagenesis, Site-Directed*/methods

  • Source: Journal of molecular biology [J Mol Biol] 2022 Apr 30; Vol. 434 (8), pp. 167199. Date of Electronic Publication: 2021 Aug 16.Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2985088R Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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Academic Journal

Intermolecular disulfide bonds between unpaired cysteines retard the C-terminal trans-cleavage of Npu DnaE.

  • Authors : Xu Y; Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.; Zhang L

Subjects: Inteins* ; Protein Splicing*; Cysteine/Cysteine/Cysteine/*chemistry

  • Source: Enzyme and microbial technology [Enzyme Microb Technol] 2018 Nov; Vol. 118, pp. 6-12. Date of Electronic Publication: 2018 Jul 03.Publisher: Elsevier Country of Publication: United States NLM ID: 8003761 Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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Academic Journal

Split-inteins and their bioapplications.

  • Authors : Li Y; R & D Services, Cytovance Biologics 800 Research Parkway, Suite 200, Oklahoma City, OK, 73104, USA. .

Subjects: Protein Splicing* ; Recombinant Proteins*/Recombinant Proteins*/Recombinant Proteins*/chemistry ; Recombinant Proteins*/Recombinant Proteins*/Recombinant Proteins*/isolation & purification

  • Source: Biotechnology letters [Biotechnol Lett] 2015 Nov; Vol. 37 (11), pp. 2121-37. Date of Electronic Publication: 2015 Jul 08.Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Print-Electronic Cited Medium: Internet

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Academic Journal

Expressed Protein Ligation without Intein.

  • Authors : Qiao Y; The Texas A&M Drug Discovery Laboratory, Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.; Yu G

Subjects: Inteins/Inteins/Inteins/*genetics ; Recombinant Proteins/Recombinant Proteins/Recombinant Proteins/*chemistry; Humans

  • Source: Journal of the American Chemical Society [J Am Chem Soc] 2020 Apr 15; Vol. 142 (15), pp. 7047-7054. Date of Electronic Publication: 2020 Apr 02.Publisher: American Chemical Society Country of Publication: United States NLM ID: 7503056 Publication Model: Print-Electronic Cited Medium: Internet

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Academic Journal

Rapid cloning and purification of proteins: gateway vectors for protein purification by self-cleaving tags.

  • Authors : Gillies AR; Department of Chemical Engineering, Princeton University, A417 Engineering Quadrangle, Olden Street, Princeton, New Jersey 08544, USA.; Hsii JF

Subjects: Genetic Vectors* ; Inteins*; Cloning, Molecular/Cloning, Molecular/Cloning, Molecular/*methods

  • Source: Biotechnology and bioengineering [Biotechnol Bioeng] 2008 Oct 01; Vol. 101 (2), pp. 229-40.Publisher: Wiley Country of Publication: United States NLM ID: 7502021 Publication Model: Print Cited Medium: Internet ISSN: 1097-0290

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Academic Journal

Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage.

  • Authors : Hong J; Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210-A Seitz Hall, Blacksburg, VA 24061, USA.; Wang Y

Subjects: Inteins*; Chromatography, Affinity/Chromatography, Affinity/Chromatography, Affinity/*methods ; Recombinant Proteins/Recombinant Proteins/Recombinant Proteins/*isolation & purification

  • Source: Journal of chromatography. A [J Chromatogr A] 2008 Jun 20; Vol. 1194 (2), pp. 150-4. Date of Electronic Publication: 2008 Apr 24.Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9318488 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0021-9673

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Academic Journal

Photo-triggered fluorescent labelling of recombinant proteins in live cells.

  • Authors : Jung D; Department of Biomedical Engineering, Dongguk University-Seoul, Pildong 3-ga, Seoul, Korea. .; Sato K

Subjects: Light*; Inteins/Inteins/Inteins/*physiology ; Maltose-Binding Proteins/Maltose-Binding Proteins/Maltose-Binding Proteins/*metabolism

  • Source: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2015 Jun 14; Vol. 51 (47), pp. 9670-3.Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 9610838 Publication Model: Print Cited Medium: Internet ISSN:

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Academic Journal

An evolved Mxe GyrA intein for enhanced production of fusion proteins.

  • Authors : Marshall CJ; Dept. of Chemical and Biological Engineering, University of Wisconsin-Madison , 1415 Engineering Drive, Madison, Wisconsin 53706, United States.; Grosskopf VA

Subjects: Directed Molecular Evolution* ; Recombinant Proteins*; DNA Gyrase/DNA Gyrase/DNA Gyrase/*chemistry

  • Source: ACS chemical biology [ACS Chem Biol] 2015 Feb 20; Vol. 10 (2), pp. 527-38. Date of Electronic Publication: 2014 Nov 24.Publisher: American Chemical Society Country of Publication: United States NLM ID: 101282906 Publication Model: Print-Electronic Cited Medium: Internet

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Academic Journal

A general purification platform for toxic proteins based on intein trans-splicing.

  • Authors : Shi C; Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, 201620, People's Republic of China.; Tarimala A

Subjects: Trans-Splicing*; Inteins/Inteins/Inteins/*genetics ; Recombinant Proteins/Recombinant Proteins/Recombinant Proteins/*isolation & purification

  • Source: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2014 Nov; Vol. 98 (22), pp. 9425-35. Date of Electronic Publication: 2014 Oct 09.Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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  • 1-10 of  126 results for ""Inteins""