Localization of Label-Retaining Cells in Murine Vocal Fold Epithelium

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Availability:
      American Speech-Language-Hearing Association (ASHA). 10801 Rockville Pike, Rockville, MD 20852. Tel: 800-638-8255; Fax: 301-571-0457; e-mail: [email protected]; Web site: http://jslhr.asha.org
    • Peer Reviewed:
      Y
    • Source:
      7
    • Subject Terms:
      Cytology; Animals
    • Accession Number:
      10.1044/1092-4388(2010/10-0267)
    • ISSN:
      1092-4388
    • Abstract:
      Purpose: Epithelial homeostasis is critical for vocal fold health, yet little is known about the cells that support epithelial self-renewal. As a known characteristic of stem cells is that they are slow-cycling in vivo, the purpose of this prospective controlled study was to identify and quantify slow-cycling cells or putative stem cells in murine vocal fold epithelium. Method: Twelve mice were administered daily intraperitoneal injections of a nucleotide dye, bromodeoxyuridine (BrdU), over 7 consecutive days. Under this pulse-chase paradigm, slow-cycling cells retain the dye (label-retaining cells; LRCs) while more rapidly cycling cells lose dye to dilution during multiple cell divisions. The percentage of label-retaining cells (%LRCs) was calculated following a chase period of 2, 4, and 8 weeks postinjections. Results: The %LRCs decreased significantly from 9.4% at 2 weeks to 3.1% at 8 weeks following injections (p less than 0.05). No statistically significant differences in the quantity of BrdU-positive cells were measured between the anterior, mid-membranous, or cartilaginous regions of the vocal fold (p greater than 0.05). Conclusion: These findings are consistent with the presence and first report of a small population of putative stem cells along the length of murine vocal fold epithelium.
    • Abstract:
      As Provided
    • Publication Date:
      2011
    • Accession Number:
      EJ936633