New Colorimetric Assay of Apurinic/Apyrimidinic (Abasic) Sites of Deoxyribonucleic Acid (DNA) Nucleotides Using Bicinchoninic Assay: A Bioanalytical Chemistry Experiment for the Undergraduate Curriculum

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  • Author(s): Stephens, Isaiah O.; Thomas, Elizabeth M. (ORCID Thomas, Elizabeth M. (ORCID 0000-0003-3514-0719)
  • Language:
    English
  • Source:
    Journal of Chemical Education. Mar 2022 99(3):1454-1459.
  • Publication Date:
    2022
  • Document Type:
    Journal Articles
    Reports - Descriptive
  • Additional Information
    • Availability:
      Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: [email protected]; Web site: http://pubs.acs.org/jchemeduc
    • Peer Reviewed:
      Y
    • Source:
      6
    • Intended Audience:
      Teachers
    • Education Level:
      Higher Education
      Postsecondary Education
    • Subject Terms:
      Science Instruction; Chemistry; Genetics; Undergraduate Study; College Science; Science Laboratories; Scientific Concepts
    • Accession Number:
      10.1021/acs.jchemed.1c00985
    • ISSN:
      0021-9584
    • Abstract:
      Apurinic/apyrimidinic (AP or abasic) sites are deoxyribonucleic acid (DNA) lesions that result from the loss of a nucleobase by hydrolysis of the "N"-glycosyl bond. AP sites are the most frequent lesions in cells and are caused by environmental and cancer therapeutic genotoxins. Herein, the bicinchoninic colorimetric assay, typically used to quantify proteins, is used to quantify abasic sites within DNA nucleotides. The abasic sites can be quantified using either a traditional colorimeter to measure absorbances at 562 nm or using hand-held cameras to measure the color change (e.g., green value). Having a rapid colorimetric method for quantifying abasic sites within DNA nucleotides would be beneficial within undergraduate teaching laboratories to introduce students to DNA structures and how DNA lesions are produced structurally.
    • Abstract:
      As Provided
    • Publication Date:
      2022
    • Accession Number:
      EJ1328746