Examination and Manipulation of Protein Surface Charge in Solution with Electrospray Ionization Mass Spectrometry

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  • Author(s): Gross, Deborah S.; Van Ryswyk, Hal
  • Language:
    English
  • Source:
    Journal of Chemical Education. Aug 2014 91(8):1240-1243.
  • Publication Date:
    2014
  • Document Type:
    Journal Articles
    Reports - Descriptive
  • Additional Information
    • Availability:
      Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: [email protected]; Web site: http://pubs.acs.org/jchemeduc
    • Peer Reviewed:
      Y
    • Source:
      4
    • Sponsoring Agency:
      National Science Foundation
    • Contract Number:
      0077540
      0922481
    • Education Level:
      Higher Education
      Postsecondary Education
    • Subject Terms:
      Science Instruction; Chemistry; Spectroscopy; Science Laboratories; Science Experiments; Molecular Structure; College Science; Undergraduate Study; Inquiry; Scientific Concepts; Teaching Methods; Laboratory Experiments
    • Accession Number:
      10.1021/ed4005886
    • ISSN:
      0021-9584
    • Abstract:
      Electrospray ionization mass spectrometry (ESI-MS) is a powerful tool for examining the charge of proteins in solution. The charge can be manipulated through choice of solvent and pH. Furthermore, solution-accessible, protonated lysine side chains can be specifically tagged with 18-crown-6 ether to form noncovalent adducts. Chemical derivatization of a protein in this fashion followed by "soft" ionization can provide a wealth of specific information regarding side chain identity, location, and surface chemistry. A variety of proteins are examined with ESI-MS under a range of conditions in this self-guided experiment, and the results are compared with those predicted from structural information available in the protein data bank.
    • Abstract:
      As Provided
    • Number of References:
      20
    • Publication Date:
      2014
    • Accession Number:
      EJ1036386