Using RNAi in C. 'elegans' to Demonstrate Gene Knockdown Phenotypes in the Undergraduate Biology Lab Setting

Association of College and Biology Educators. Web site: http://acube.org

Y

5

Biology; College Science; Science Instruction; Science Laboratories; Genetics; Undergraduate Students; Molecular Biology; Science Activities; Hands on Science; Microbiology; Assignments; Biotechnology

1539-2422

RNA interference (RNAi) is a powerful technology used to knock down genes in basic research and medicine. In 2006 RNAi technology using "Caenorhabditis elegans" ("C. elegans") was awarded the Nobel Prize in medicine and thus students graduating in the biological sciences should have experience with this technology. However, students struggle conceptually with the molecular biology behind the RNAi technology and find the technology difficult to grasp. To this end, we have provided a simple, streamlined and inexpensive RNAi procedure using "C. elegans" that can be adopted in upper level biology classes. By using an unknown RNAi-producing bacteria, students perform novel techniques, observe and determine which mystery gene was knocked down based on phenotype and experience a new research organism. By bringing this technology to the undergraduate lab bench, the gap between blackboard concept and proof of concept can be bridged.

As Provided

9

2014

EJ1020522