Changes in the location of polyphenol oxidase in potato (Solanum tuberosum L.) tuber during cell death in response to impact injury: comparison with wound tissue.

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  • Additional Information
    • Source:
      Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Print Cited Medium: Print ISSN: 0032-0935 (Print) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin, New York, Springer-Verlag [etc.]
    • Subject Terms:
      Catechol Oxidase/*analysis ; Solanum tuberosum/*enzymology; Cell Death ; Immunohistochemistry
    • Abstract:
      In order to elucidate the nature of the response of potato to impact injury at the biochemical level, changes in the location of the enzyme responsible for the discoloration, polyphenol oxidase, were determined using immunogold location with an antibody specific for potato tuber polyphenol oxidase. Tissue printing revealed that the enzyme was distributed throughout the tuber. Following impact injury, both tissue printing and quantitative electron microscopy indicated that there was no increase in the level of the enzyme although there was subcellular redistribution of polyphenol oxidase. This redistribution was first apparent at 12 h after impact, as determined by the use of confocal immunolocation, and coincided with loss of membrane integrity. These changes were examined in parallel with a number of stress-related parameters in both impact and wound responses. Wounding was accompanied by active gene expression and protein synthesis, leading to metabolic activity and tissue repair. In contrast, the bruising response was characterised by a limited active response and vital-staining methods indicated that after 16 h the tissue undergoes cell death.
    • Accession Number:
      EC 1.10.3.1 (Catechol Oxidase)
    • Publication Date:
      Date Created: 19990210 Date Completed: 19990325 Latest Revision: 20061115
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/s004250050504
    • Accession Number:
      9951737