Regulation of fibronectin gene expression by cyclic AMP and phorbol myristate acetate in HT-1080 human fibrosarcoma cells.

Publisher: Nature Publishing Group Country of Publication: United States NLM ID: 9607880 Publication Model: Print Cited Medium: Print ISSN: 1226-3613 (Print) Linking ISSN: 12263613 NLM ISO Abbreviation: Exp Mol Med Subsets: MEDLINE

Publication: Jan. 2013- : New York : Nature Publishing Group
Original Publication: Seoul : Korean Society of Medical Biochemistry and Molecular Biology, 1996-

Gene Expression Regulation*; Bucladesine/*pharmacology ; Fibronectins/*genetics ; Fibrosarcoma/*genetics ; Tetradecanoylphorbol Acetate/*pharmacology; Blotting, Northern ; Bucladesine/antagonists & inhibitors ; Enzyme-Linked Immunosorbent Assay ; Fibronectins/metabolism ; Humans ; Luciferases/metabolism ; Precipitin Tests ; Promoter Regions, Genetic ; RNA, Messenger/metabolism ; Transfection ; Tumor Cells, Cultured ; beta-Galactosidase/metabolism

We studied the regulation of fibronectin (FN) gene expression by cAMP and phorbol-12-myristate-13-acetate (PMA) in HT-1080 human fibrosarcoma cells. Dibutyryl cAMP increased FN synthesis and mRNA levels, while PMA inhibited the cAMP-induced FN synthesis. In transient transfection assays, cAMP increased FN promoter activity, while PMA paradoxically enhanced the cAMP-induced promoter activity. Stable transfection experiments, however, showed that neither cAMP or PMA alone nor together affected FN promoter activity. These results suggest that PMA antagonizes the cAMP-induced FN gene expression and that both the action of cAMP and the inhibition of its action by PMA may occur at the posttranscriptional level in HT-1080 cells.

0 (Fibronectins)
0 (RNA, Messenger)
63X7MBT2LQ (Bucladesine)
EC 1.13.12.- (Luciferases)
EC 3.2.1.23 (beta-Galactosidase)
NI40JAQ945 (Tetradecanoylphorbol Acetate)

Date Created: 19990123 Date Completed: 19990322 Latest Revision: 20131121

20231215

10.1038/emm.1998.35

9894155