Formation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by PHA synthase from Ralstonia eutropha.

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  • Author(s): Dennis D;Dennis D; McCoy M; Stangl A; Valentin HE; Wu Z
  • Source:
    Journal of biotechnology [J Biotechnol] 1998 Oct 08; Vol. 64 (2-3), pp. 177-86.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8411927 Publication Model: Print Cited Medium: Print ISSN: 0168-1656 (Print) Linking ISSN: 01681656 NLM ISO Abbreviation: J Biotechnol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam : Elsevier Science Publishers, c1984-
    • Subject Terms:
      Acyltransferases/*metabolism ; Cupriavidus necator/*enzymology; 3-Hydroxybutyric Acid/metabolism ; Alcohol Oxidoreductases/metabolism ; Caproates/metabolism ; Carbon/metabolism ; Klebsiella pneumoniae/genetics ; Klebsiella pneumoniae/growth & development ; Recombination, Genetic
    • Abstract:
      The acetoacetyl-CoA reductase and the polyhydroxyalkanoate (PHA) synthase from Ralstonia eutropha (formerly Alcaligenes eutrophus) were expressed in Escherichia coli, Klebsiella aerogenes, and PHA-negative mutants of R. eutropha and Pseudomonas putida. While expression in E. coli strains resulted in the accumulation of poly(3-hydroxybutyrate) [PHB], strains of R. eutropha, P. putida and K. aerogenes accumulated poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [poly(3HB-co-3HHx)] when even chain fatty acids were provided as carbon source, and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)] when odd chain fatty acids were provided as carbon source. This suggests that fatty acid degradation can be directly accessed employing only the acetoacetyl-CoA reductase and the PHA synthase. This is also the first proof that the PHA synthase from R. eutropha can incorporate 3-hydroxyhexanoate (3HHx) into PHA and has, therefore, a broader substrate specificity than previously described.
    • Accession Number:
      0 (Caproates)
      0 (poly(3-hydroxybutyrate-co-3-hydroxyhexanoate))
      7440-44-0 (Carbon)
      EC 1.1.- (Alcohol Oxidoreductases)
      EC 1.1.1.36 (acetoacetyl-CoA reductase)
      EC 2.3.- (Acyltransferases)
      EC 2.3.1.- (poly(3-hydroxyalkanoic acid) synthase)
      TZP1275679 (3-Hydroxybutyric Acid)
    • Publication Date:
      Date Created: 19981120 Date Completed: 19981218 Latest Revision: 20190915
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/s0168-1656(98)00110-2
    • Accession Number:
      9821674