An endothelial growth factor involved in rat renal development.

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  • Author(s): Oliver JA;Oliver JA; Al-Awqati Q
  • Source:
    The Journal of clinical investigation [J Clin Invest] 1998 Sep 15; Vol. 102 (6), pp. 1208-19.
  • Publication Type:
    Journal Article; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Society for Clinical Investigation Country of Publication: United States NLM ID: 7802877 Publication Model: Print Cited Medium: Print ISSN: 0021-9738 (Print) Linking ISSN: 00219738 NLM ISO Abbreviation: J Clin Invest Subsets: MEDLINE
    • Publication Information:
      Publication: 1999- : Ann Arbor, MI : American Society for Clinical Investigation
      Original Publication: New Haven [etc.] American Society for Clinical Investigation.
    • Subject Terms:
    • Abstract:
      In the kidney, there is a close and intricate association between epithelial and endothelial cells, suggesting that a complex reciprocal interaction may exist between these two cell types during renal ontogeny. Thus, we examined whether metanephrogenic mesenchymal cells secrete endothelial mitogens. With an endothelial mitogenic assay and sequential chromatography of the proteins in the media conditioned by a cell line of rat metanephrogenic mesenchymal cells (7.1.1 cells), we isolated a protein whose amino acid analysis identified it as hepatoma-derived growth factor (HDGF). Media conditioned with Cos-7 cell transfected with HDGF cDNA stimulated endothelial DNA synthesis. With immunoaffinity purified antipeptide antibodies, we found that HDGF was widely distributed in the renal anlage at early stages of development but soon concentrated at sites of active morphogenesis and, except for some renal tubules, disappeared from the adult kidney. From a 7.1.1 cells cDNA library, a clone of most of the translatable region of HDGF was obtained and used to synthesize digoxigenin-labeled riboprobes. In situ hybridization showed that during kidney development mRNA for HDGF was most abundant at sites of nephron morphogenesis and in ureteric bud cells while in the adult kidney transcripts disappeared except for a small population of distal tubules. Thus, HDGF is an endothelial mitogen that is present in embryonic kidney, and its expression is synchronous with nephrogenesis.
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    • Grant Information:
      DK-46934 United States DK NIDDK NIH HHS
    • Accession Number:
      0 (Culture Media, Conditioned)
      0 (DNA, Complementary)
      0 (Endothelial Growth Factors)
      0 (Growth Substances)
      0 (Intercellular Signaling Peptides and Proteins)
      0 (Mitogens)
      0 (Recombinant Proteins)
      0 (hepatoma-derived growth factor)
    • Publication Date:
      Date Created: 19980917 Date Completed: 19981013 Latest Revision: 20220316
    • Publication Date:
      20240829
    • Accession Number:
      PMC509104
    • Accession Number:
      10.1172/JCI785
    • Accession Number:
      9739055