Ouabain suppresses ATP elevation in response to fuel secretagogues in pancreatic islets.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
    • Publication Information:
      Publication: <2002- >: San Diego, CA : Elsevier
      Original Publication: New York, Academic Press.
    • Subject Terms:
      Adenosine Triphosphate/*metabolism ; Islets of Langerhans/*drug effects ; Islets of Langerhans/*metabolism ; Ouabain/*pharmacology; Animals ; Enzyme Inhibitors/pharmacology ; Glucose/pharmacology ; Glyceraldehyde/pharmacology ; In Vitro Techniques ; Keto Acids/pharmacology ; Kinetics ; Male ; Rats ; Rats, Wistar ; Sodium-Potassium-Exchanging ATPase/antagonists & inhibitors
    • Abstract:
      The alterations of the ATP concentration in response to fuel secretagogues such as glucose, glyceraldehyde, and ketoisocaproate (KIC) were investigated in a single islet. The intraislet ATP concentration was transiently elevated and then decreased to a level slightly higher than basal. To asses the ATP content under conditions of reduced ATP consumption, the Na-K pump blocker ouabain was used. The elevation of ATP concentration was found unexpectedly to be suppressed under ouabain in the islet, even when incubated with any of the secretagogues. High glucose did not elevate the intracellular creatine phosphate during incubation with ouabain. Since the suppression rate for the intraislet ATP elevation was considerably smaller with KIC than with glyceraldehyde and glucose, we conclude that ouabain inhibits ATP production, at least in part, in the glycolytic pathway through a feedback mechanism.
    • Accession Number:
      0 (Enzyme Inhibitors)
      0 (Keto Acids)
      367-47-5 (Glyceraldehyde)
      5ACL011P69 (Ouabain)
      816-66-0 (alpha-ketoisocaproic acid)
      8L70Q75FXE (Adenosine Triphosphate)
      EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
      IY9XDZ35W2 (Glucose)
    • Publication Date:
      Date Created: 19980910 Date Completed: 19981002 Latest Revision: 20191210
    • Publication Date:
      20231215
    • Accession Number:
      10.1006/bbrc.1998.9240
    • Accession Number:
      9731226