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Rab35 regulates pseudopodia extension of breast cancer cells in response to extracellular matrix.
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- Additional Information
- Abstract:
Pseudopodia extension in directional movement remains poorly defined in breast cancer cells. We showed that the modified Boyden chamber assay could be used for pseudopodia extension and isolation from migrating breast cancer cells in response to ECM (collagen type I). Rab35, enriched in pseudopodia of breast cancer cells, promoted pseudopodia extension in response to ECM. Dominant negative Rab35 mutant or Rab35 shRNA significantly decreased ECM-induced pseudopodia extension. Rab35 was strongly associated with fascin in MDA-MB-231 and MCF-7 cells. Rab35 mRNAs were significantly enriched in pseudopodia of breast cancer cells in response to ECM stimuli. Blocking microtubule assembly by microtubule-depolymerizing agent nocodazole significantly (18.9±8.4% and 27.8±8.2%, p<0.05). Statistical test was one-way ANOVA. Conclusion A physiologically relevant concentration of the flavonoid quercetin significantly decreases the spontaneous tone of intramural coronary arterioles. A substantial part of this vasorelaxation effect is connected to production of vasodilatory endogenous prostanoids while an endothelial NO-mediated mechanism seems to be negligible. These observations suggest the possibility that quercetin appearing in blood plasma from the food induces the operation of a coronary vasodilatory mechanism whose effectiveness is commeasurable with that of the known endothelial actions. (Supported by OTKA 32019, 42670, the Hungarian Hypertension Society, and the Hungarian Kidney Foundation) [ABSTRACT FROM AUTHOR]
- Abstract:
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