[Endonucleolysis of chromatin in rat thymus and spleen lymphocytes after irradiation].

Endonukleoliz khromatynu u limfotsytakh selezinky ta tymusa shchuriv pislia oprominennia.

Publisher: Izdatelstvo Naukova Dumka Country of Publication: Ukraine NLM ID: 7804246 Publication Model: Print Cited Medium: Print ISSN: 0201-8470 (Print) Linking ISSN: 02018470 NLM ISO Abbreviation: Ukr Biokhim Zh (1978) Subsets: MEDLINE

Original Publication: Kiev : Izdatelstvo Naukova Dumka

Chromatin/*radiation effects ; Endodeoxyribonucleases/*metabolism ; Spleen/*radiation effects ; T-Lymphocytes/*radiation effects ; Thymus Gland/*radiation effects; Animals ; Chromatin/chemistry ; DNA/analysis ; DNA Fragmentation/radiation effects ; Electrophoresis, Agar Gel ; Enzyme Activation/radiation effects ; Nucleosomes/radiation effects ; Rats ; Rats, Wistar ; Spleen/cytology ; T-Lymphocytes/enzymology ; Thymus Gland/cytology ; Whole-Body Irradiation

The level of soluble DNA fragments (polydesoxynucleotides) in thymus and spleen lymphocytes is enhanced 12 h after the whole-body X-ray irradiation in dose of 0.5 and 1 Gy. The level of Ca2+, Mg(2+)-DNAase activity in lysed cells extracts, measured with calf spleen DNA, was enhanced in the region of 0.1-0.5 mM CaCl2 in incubation medium and decreased at 5 mM CaCl2. Monitoring of DNA fragmentation by electrophoresis in agarose gels showed the presence of fragments with approximately 200, 400, 800 bp length. X-ray induced DNA fragmentation is supposed to be associated with Ca2+, Mg(2+)-endonuclease activation.

0 (Chromatin)
0 (Nucleosomes)
9007-49-2 (DNA)
EC 3.1.- (Endodeoxyribonucleases)
EC 3.1.21.- (calcium magnesium dependent endodeoxyribonuclease)

Date Created: 19970701 Date Completed: 19980701 Latest Revision: 20150505

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