Functional expression of the bovine herpesvirus 1 alkaline deoxyribonuclease (UL12) in Escherichia coli.

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  • Author(s): Chung YT;Chung YT; Hsu W
  • Source:
    Archives of virology [Arch Virol] 1996; Vol. 141 (12), pp. 2457-64.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7506870 Publication Model: Print Cited Medium: Print ISSN: 0304-8608 (Print) Linking ISSN: 03048608 NLM ISO Abbreviation: Arch Virol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Wien, New York, Springer-Verlag.
    • Subject Terms:
      Deoxyribonucleases/*genetics ; Deoxyribonucleases/*metabolism ; Herpesvirus 1, Bovine/*genetics; Chromatography, Affinity ; Chromosome Mapping ; Deoxyribonucleases/isolation & purification ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Gene Expression ; Genes, Viral ; Genome, Viral ; Herpesviridae/genetics ; Open Reading Frames ; Plasmids ; Recombination, Genetic ; Thiogalactosides/metabolism
    • Abstract:
      Sequence analysis within the unique long segment of the bovine herpesvirus 1 (BHV-1; infectious bovine rhinotracheitis virus) genome identified an open reading frame whose deduced protein product of 487 amino acids exhibited homology to alkaline deoxyribonucleases (DNases) of other herpesviruses. To determine this BHV-1 gene product has nuclease activity, the gene designated UL12 was inserted into the vector pET-28a(+) and expressed in Escherichia coli as an oligohistidine-tagged protein. Upon induction with isopropyl beta-D-thiogalactopyranoside E. coli BL21 (DE3) [pLysS] cells carrying this recombinant plasmid produced a 57-kDa protein, the molecular mass of which was in accordance with the prediction from the DNA sequence. The recombinant UL12 protein purified by nickel-chelating affinity chromatography exhibited both exonuclease and endonuclease activity, each with an alkaline pH optimum.
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    • Accession Number:
      0 (Thiogalactosides)
      EC 3.1.- (Deoxyribonucleases)
    • Publication Date:
      Date Created: 19960101 Date Completed: 19980421 Latest Revision: 20200304
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/BF01718643
    • Accession Number:
      9526549