[Comparison of the DNA-GEN Probe PACE 2 method and the LCR method for the detection of Chlamydia trachomatis in the female genital tract].

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  • Author(s): Binder T;Binder T; Hrusková H; Drazdáková M; Kuklová I
  • Source:
    Ceska gynekologie [Ceska Gynekol] 1997 Jun; Vol. 62 (3), pp. 131-3.
  • Publication Type:
    Comparative Study; English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    Czech
  • Additional Information
    • Transliterated Title:
      Srovnávací studie metodik DNA-GEN probe PACE 2 a LCR k průkazu Chlamydia trachomatis v zenském genitálním ústrojí.
    • Source:
      Publisher: Ceská lékarska spolecnost J. E. Purkyne Country of Publication: Czech Republic NLM ID: 9423768 Publication Model: Print Cited Medium: Print ISSN: 1210-7832 (Print) Linking ISSN: 12107832 NLM ISO Abbreviation: Ceska Gynekol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Praha : Ceská lékarska spolecnost J. E. Purkyne, c1994-
    • Subject Terms:
      In Situ Hybridization, Fluorescence* ; Nucleic Acid Amplification Techniques*; Cervix Uteri/*microbiology ; Chlamydia trachomatis/*isolation & purification; Adult ; Chlamydia Infections/diagnosis ; Chlamydia trachomatis/genetics ; DNA Ligases ; Female ; Humans ; Polymerase Chain Reaction ; Pregnancy ; Pregnancy Complications, Infectious/diagnosis ; RNA, Ribosomal/analysis ; Sensitivity and Specificity
    • Abstract:
      Objective: Comparison of two methods of so-called rapid assessment of Chlamydia trachomatis in the urogenital system, i.e. the hybridization method GEN-Probe PACE 2 and the amplification LCR (ligase chain reaction) method. In 1995-1996 the authors made a screening examination to detect Chlamydiae in the urogenital tract by means of the hybridization method GEN-Probe PACE 2 and the amplification LCR method in selected groups of patients of the Second Gynaecological and Obstetric Clinic, First Faculty Hospital, Prague. The methods were evaluated as to their sensitivity. The examinations of the samples were made in the serological laboratory of the First Dermatological Clinic of the First Faculty Hospital Charles University, Prague. The amplification method is twice as sensitive as the hybridization method.
    • Accession Number:
      0 (RNA, Ribosomal)
      EC 6.5.1.- (DNA Ligases)
    • Publication Date:
      Date Created: 19970601 Date Completed: 19980108 Latest Revision: 20161206
    • Publication Date:
      20240829
    • Accession Number:
      9424251