Cellular Profile of Cytokine Production in a Patient with Visceral Leishmaniasis: γδ⁺ T Cells Express Both Type 1 Cytokines and Interleukin-10.

Abstract The cytokine profile of CD4+ , CD8+ T cells, γδ+ T cells and natural killer (NK) cells (CD94+ CD3– ) was studied in a patient with visceral leishmaniasis (VL). The otherwise healthy, human immunodeficiency virus-negative patient acquired the disease in Tuscany, Italy. Diagnosis was made by demonstration of high concentrations of antibodies against Leishmania antigens in serum. Flow cytometry for the detection of intracellular interferon-γ (IFN-γ), interleukin (IL)-2, IL-4, IL-6, IL-10, IL-13 and tumour necrosis factor (TNF)-α expression in peripheral blood mononuclear cells stimulated with phorbol 12-myristate 13-acetate and ionomycin was performed, followed by treatment with liposomal amphotericin B. CD4+ cells were identified as major cytokine-expressing cells, capable of producing both type 1 and type 2 cytokines. A high frequency of IL-4- and IL-13-expressing CD8+ cells was noted. NK cells and γδ+ T cells, thought to be involved in innate host defences against Leishmania , expressed IFN-γ and TNF-α. Ten per cent of γδ+ T cells expressed IL-10, predominantly together with IFN-γ, suggesting additional immune-regulatory roles for this T-cell subset in VL. [ABSTRACT FROM AUTHOR]

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