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Incomplete processing of type II procollagen by a rat chondrosarcoma cell line.
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- Author(s): Fernandes RJ;Fernandes RJ; Schmid TM; Harkey MA; Eyre DR
- Source:
European journal of biochemistry [Eur J Biochem] 1997 Jul 15; Vol. 247 (2), pp. 620-4.
- Publication Type:
Journal Article; Research Support, U.S. Gov't, P.H.S.
- Language:
English
- Additional Information
- Source:
Publisher: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Country of Publication: England NLM ID: 0107600 Publication Model: Print Cited Medium: Print ISSN: 0014-2956 (Print) Linking ISSN: 00142956 NLM ISO Abbreviation: Eur J Biochem Subsets: MEDLINE
- Publication Information:
Publication: -2004: Oxford, UK : Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Original Publication: Berlin, New York, Springer.
- Subject Terms:
- Abstract:
The Swarm rat chondrosarcoma cell line, RCS-LTC, deposits an extracellular matrix that contains the typical type II, IX, and XI collagen phenotype of hyaline cartilage, but the fibrils appear abnormally thin. By N-terminal sequence analysis, the type II collagen from the matrix was shown to have retained its N-propeptides with no evidence of normal processing to type II collagen. Amplification and sequencing of cDNA prepared from the pro alpha1(II) mRNA of these cells showed a normal N-propeptide cleavage site. Furthermore, the type II N-procollagen could be processed to type II collagen by incubation with culture medium from normal chondrocytes. The findings indicate that the RCS-LTC cell line fails to express an active type II procollagen N-proteinase and, therefore, offers a useful culture system in which to study the role of N-propeptide removal in fibrillogenesis.
- Grant Information:
R01 AR036794 United States AR NIAMS NIH HHS; R37 AR037318 United States AR NIAMS NIH HHS; AR 37318 United States AR NIAMS NIH HHS; AR 39239 United States AR NIAMS NIH HHS
- Molecular Sequence:
GENBANK L48440
- Accession Number:
0 (DNA, Complementary)
0 (Procollagen)
0 (RNA, Messenger)
0 (Recombinant Proteins)
9007-34-5 (Collagen)
EC 3.4.24.14 (Procollagen N-Endopeptidase)
- Publication Date:
Date Created: 19970715 Date Completed: 19970908 Latest Revision: 20190620
- Publication Date:
20250114
- Accession Number:
10.1111/j.1432-1033.1997.00620.x
- Accession Number:
9266705
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