Association of IgA nephropathy with T-cell receptor constant alpha chain gene polymorphism.

Publisher: W.B. Saunders Country of Publication: United States NLM ID: 8110075 Publication Model: Print Cited Medium: Print ISSN: 0272-6386 (Print) Linking ISSN: 02726386 NLM ISO Abbreviation: Am J Kidney Dis Subsets: MEDLINE

Publication: Philadelphia Pa : W.B. Saunders
Original Publication: New York, N.Y. : Grune & Stratton, c1981-

Polymorphism, Genetic*; Glomerulonephritis, IGA/*genetics ; Receptors, Antigen, T-Cell, alpha-beta/*genetics; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Polymorphism, Restriction Fragment Length

T-cell receptor (TCR) proteins recognize a complex of an antigen-derived peptide bound to the cell surface products of the major histocompatibility complex (MHC) that could be of importance in the immunopathogenesis of IgA nephropathy (IgAN). Previous studies found no difference on TCR constant beta chain gene frequencies in IgAN compared with control. Yet no study on the TCR alpha gene in IgAN was reported. We studied the TCR C alpha gene polymorphisms by restriction fragment length polymorphism (RFLP) in 53 patients with IgAN and in comparison with 67 healthy controls. The patients were also classified into different histopathological grading (I, II, and III with increasing histological severity) and renal functions. The extracted DNA were digested with Taq I enzymes and probed with a full-length TCR-alpha cDNA clone p1.2alpha probe. A 7-kb C-alpha Taq 1 fragment is found in 32 of 53 patients (60.3%) compared with 26 of 67 controls (38.8%) (P < 0.05). There was no association of any polymorphic fragment, including the 7-kb fragment, with either the histological grading or renal function. It is concluded that the TCR C-alpha gene is associated with IgAN but not with the prognosis of the disease.

0 (Receptors, Antigen, T-Cell, alpha-beta)

Date Created: 19970801 Date Completed: 19970903 Latest Revision: 20190815

20240829

10.1016/s0272-6386(97)90061-5

9261038