High resolution HLA-C typing by PCR-SSP: identification of allelic frequencies and linkage disequilibria in 604 unrelated random UK Caucasoids and a comparison with serology.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Source:
      Publisher: Wiley Blackwell Country of Publication: England NLM ID: 0331072 Publication Model: Print Cited Medium: Print ISSN: 0001-2815 (Print) Linking ISSN: 00012815 NLM ISO Abbreviation: Tissue Antigens Subsets: MEDLINE
    • Publication Information:
      Publication: <2015>: [Oxford] : Wiley Blackwell
      Original Publication: 1971- : Copenhagen, Munksgaard.
    • Subject Terms:
    • Abstract:
      Recent evidence indicates that HLA-C molecules are biologically relevant by eliciting T-cell responses and exerting control over NK cell function. In addition, HLA-C is associated with susceptibility to various diseases, notably psoriasis vulgaris. Clarification of the full biological roles for HLA-C has however proved difficult because detection of HLA-C antigens by complement mediated cytotoxicity using alloantisera is inefficient. Up to 50% of individuals in every race have serologically undetectable HLA-C locus antigens due to a combination of relatively low expression, lack of serological reagents and a lack of information about the distribution of the HLA-C blank alleles. Recently, amplification of DNA using sequence-specific primers (PCR-SSP) has proved a reliable, accurate and rapid method for medium resolution HLA-C typing. We have now developed high resolution HLA-C typing by PCR-SSP utilizing allele and group-specific PCR-SSP reactions which can identify all HLA-C alleles (except non-coding change alleles) in most heterozygous combinations. Using this system we have typed 604 unrelated United Kingdom Caucasoids to generate accurate frequency and linkage disequilibrium data. To assess the validity of serology for HLA-C, PCR-SSP typings for 527 out of the 604 individuals were compared to serology. We find that the frequency of many HLA-C antigens has been underestimated by serology and some antigens such as Cw6 are consistently assigned incorrectly by serology. The overall discrepancy rate between serology and SSP was high at 37% (195/527). High-resolution HLA-C typing of 112 International Histocompatibility Workshop cell lines has also been performed.
    • Comments:
      Republished from: Tissue Antigens. 1996 Dec;48(6):680-91. (PMID: 9008311)
    • Accession Number:
      0 (HLA-C Antigens)
    • Publication Date:
      Date Created: 19970701 Date Completed: 19971021 Latest Revision: 20221207
    • Publication Date:
      20221213
    • Accession Number:
      10.1111/j.1399-0039.1997.tb02847.x
    • Accession Number:
      9243768