Restrictive clonal allocation in the chimeric mouse brain.

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  • Author(s): Kuan CY;Kuan CY; Elliott EA; Flavell RA; Rakic P
  • Source:
    Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1997 Apr 01; Vol. 94 (7), pp. 3374-9.
  • Publication Type:
    Journal Article; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Print ISSN: 0027-8424 (Print) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : National Academy of Sciences
    • Subject Terms:
      Chimera* ; Clone Cells*; Brain/*pathology; Animals ; Brain/growth & development ; Cell Lineage ; Embryo, Mammalian/cytology ; Mice ; Mice, Inbred C57BL ; Polymerase Chain Reaction ; Stem Cells/cytology
    • Abstract:
      Whether, and to what extent, lineage restriction contributes to the organization of the mammalian brain remains unclear. Here we address this issue by examining the distribution of clonally related cells in chimeric mice generated by injecting genetically tagged embryonic stem (ES) cells into blastocyst embryos. Our examination of postnatal chimeric brains revealed that the vast majority of labeled ES cell descendents were confined within a different subset of brain regions in each animal. Moreover, the deployment of labeled cells in different brain regions was distinctive. The pattern of ordered and binomial colonization suggested that early diversified founder cells may constrain the fates of their descendants through a restriction of dispersion. In addition, the symmetrical distribution of ES cell descendants suggests that bilaterally corresponding structures may arise from a common set of progenitor cells. Finally, clones of cells formed a continuous band within the deep strata of the neocortex. This later finding in conjunction with the radial distribution of clones in remaining layers observed in previous studies indicates that the cerebral neocortex may derive from two groups of founder cells, which is consistent with the hypothesis of dual phylogenetic origins of the mammalian cerebral cortex.
    • Comments:
      Comment in: Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2800-4. doi: 10.1073/pnas.94.7.2800. (PMID: 9096300)
    • References:
      Trends Neurosci. 1988 Feb;11(2):46-50. (PMID: 2465597)
      Science. 1992 Jan 24;255(5043):434-40. (PMID: 1734520)
      Neuron. 1989 Dec;3(6):705-13. (PMID: 2642015)
      Nature. 1993 Apr 15;362(6421):636-8. (PMID: 8464514)
      Science. 1988 Jul 8;241(4862):170-6. (PMID: 3291116)
      Development. 1995 Mar;121(3):803-12. (PMID: 7720584)
      Nature. 1990 Aug 30;346(6287):847-50. (PMID: 2202907)
      Neuroscience. 1993 Apr;53(4):1035-47. (PMID: 7685067)
      Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1184-8. (PMID: 1741373)
      Dev Biol. 1993 Mar;156(1):49-68. (PMID: 8449373)
      Trends Neurosci. 1993 Nov;16(11):472-9. (PMID: 7507621)
      J Exp Med. 1994 Feb 1;179(2):681-94. (PMID: 8294875)
      Dev Biol. 1992 Jul;152(1):133-44. (PMID: 1628751)
      Neuron. 1988 Oct;1(8):635-47. (PMID: 3272182)
      Genomics. 1995 Jul 20;28(2):154-62. (PMID: 8530021)
      Development. 1991 Nov;113(3):891-911. (PMID: 1821858)
      J Virol. 1988 Oct;62(10):3795-801. (PMID: 3418785)
      Cell. 1986 Jul 4;46(1):19-29. (PMID: 3013418)
      Neuron. 1995 Aug;15(2):311-21. (PMID: 7646888)
      Nature. 1993 Apr 15;362(6421):638-40. (PMID: 8464515)
      Dev Growth Differ. 1991 Dec;33(6):571-578. (PMID: 37282276)
      Science. 1987 May 15;236(4803):851-4. (PMID: 3576203)
      Exp Neurol. 1990 Jul;109(1):131-9. (PMID: 2358057)
      Brain Res Dev Brain Res. 1996 Mar 29;92(1):91-100. (PMID: 8861727)
      Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11323-7. (PMID: 8524778)
      Proc Natl Acad Sci U S A. 1976 Oct;73(10):3628-32. (PMID: 824647)
      J Neurogenet. 1984 Dec;1(4):275-88. (PMID: 6536730)
      Nature. 1961 Nov 25;192:766-8. (PMID: 17533671)
      Dev Biol. 1990 Sep;141(1):70-83. (PMID: 2167859)
      Nature. 1992 Dec 24-31;360(6406):737-41. (PMID: 1361214)
      J Neurosci. 1983 May;3(5):1019-38. (PMID: 6842280)
      Annu Rev Neurosci. 1981;4:301-50. (PMID: 7013637)
      Development. 1994 Feb;120(2):265-76. (PMID: 8149908)
      J Hered. 1981 Jan-Feb;72(1):3-5. (PMID: 7204939)
      J Neurosci. 1982 Oct;2(10):1494-8. (PMID: 7119869)
      Cell. 1991 Aug 23;66(4):713-29. (PMID: 1878969)
      PCR Methods Appl. 1994 Jun;3(6):S113-22. (PMID: 7920232)
      EMBO J. 1986 Dec 1;5(12):3133-42. (PMID: 3102226)
    • Grant Information:
      R01 NS014841 United States NS NINDS NIH HHS; NS14841 United States NS NINDS NIH HHS
    • Publication Date:
      Date Created: 19970401 Date Completed: 19970508 Latest Revision: 20240914
    • Publication Date:
      20240914
    • Accession Number:
      PMC20377
    • Accession Number:
      10.1073/pnas.94.7.3374
    • Accession Number:
      9096401