T cell responses to natural human proteins in primary biliary cirrhosis.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 0057202 Publication Model: Print Cited Medium: Print ISSN: 0009-9104 (Print) Linking ISSN: 00099104 NLM ISO Abbreviation: Clin Exp Immunol Subsets: MEDLINE
    • Publication Information:
      Publication: 2022- : Oxford : Oxford University Press
      Original Publication: Oxford : Blackwell Scientific Publications
    • Subject Terms:
      Autoantigens/*immunology ; Liver Cirrhosis, Biliary/*immunology ; Proteins/*immunology ; Pyruvate Dehydrogenase Complex/*immunology ; T-Lymphocytes/*immunology; Adult ; Aged ; Dihydrolipoyllysine-Residue Acetyltransferase ; Female ; Humans ; Middle Aged ; Peptides/immunology
    • Abstract:
      The study of T cell responses to autoantigens in human autoimmunity has been hampered by difficulties, firstly in identifying significant autoantigens, and secondly in the purification of authentic human proteins in sufficient quantities to allow characterization of antigen-specific T cell responses. In this study we have purified a human autoantigen, pyruvate dehydrogenase, retaining its enzymatic activity, and characterized autoreactive T cell responses to it in a human autoimmune disease, primary biliary cirrhosis. T cell responses to a mixture of the E2 and protein X subunits of human pyruvate dehydrogenase complex are seen in most affected patients, but in only a small minority of normal and chronic liver disease controls. By contrast, responses to whole pyruvate dehydrogenase complex occur with equal frequency in both groups. This suggests that responses to the E2 component/protein X of pyruvate dehydrogenase complex play a role in the pathogenesis of primary biliary cirrhosis. The availability of significant quantities of the human autoantigen in primary biliary cirrhosis makes this condition an interesting model in which to study true autoreactive human T cell responses.
    • Grant Information:
      United Kingdom Wellcome Trust
    • Accession Number:
      0 (Autoantigens)
      0 (PDHX protein, human)
      0 (Peptides)
      0 (Proteins)
      0 (Pyruvate Dehydrogenase Complex)
      EC 2.3.1.12 (Dihydrolipoyllysine-Residue Acetyltransferase)
    • Publication Date:
      Date Created: 19970301 Date Completed: 19970404 Latest Revision: 20190512
    • Publication Date:
      20231215
    • Accession Number:
      PMC1904611
    • Accession Number:
      10.1046/j.1365-2249.1997.3101202.x
    • Accession Number:
      9067533