A single amino acid change in the para sodium channel protein is associated with knockdown-resistance (kdr) to pyrethroid insecticides in German cockroach.

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  • Author(s): Dong K;Dong K
  • Source:
    Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 1997 Feb; Vol. 27 (2), pp. 93-100.
  • Publication Type:
    Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: England NLM ID: 9207282 Publication Model: Print Cited Medium: Print ISSN: 0965-1748 (Print) Linking ISSN: 09651748 NLM ISO Abbreviation: Insect Biochem Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: <2003->: Amsterdam : Elsevier Science
      Original Publication: Oxford [England] ; New York : Pergamon Press, c1992-
    • Subject Terms:
      Cockroaches*/genetics ; Insecticide Resistance* ; Pyrethrins*; Insect Proteins/*genetics ; Sodium Channels/*genetics; Amino Acid Sequence ; Animals ; Base Sequence ; DNA, Complementary ; Insect Proteins/metabolism ; Leucine ; Molecular Sequence Data ; Phenylalanine ; Sequence Homology, Amino Acid ; Sodium Channels/metabolism
    • Abstract:
      Previous genetic and pharmacological studies suggest that knockdown-resistance (kdr) to pyrethroid insecticides likely results from a mutation(s) in the para sodium channel gene. In this study, para sodium channel genes from an insecticide-susceptible German cockroach strain, CSMA, and a kdr-type German cockroach strain, Ectiban-R, were cloned and sequenced. Comparison of the nucleotide sequences of paraCSMA and paraEctiban-R revealed two nucleotide differences at nt 1491 and nt 2979, respectively. Only the difference at nt 2979 (G in paraCSMA and C in paraEctiban-R) resulted in an amino acid change (Leu993 in CSMA and Phe993 in Ectiban-R). Leu993/Phe993 is located in the highly conserved membrane-spanning segment 6 of domain II (IIS6). Furthermore, reverse transcription-polymerase chain reaction (RT-PCR) sequence analysis using another two insecticide-susceptible and one kdr German cockroach strain, revealed that C2979 was specifically associated with kdr resistant strains, whereas only G2979 was present in all susceptible strains. These results suggest that a single amino acid change from Leu993 to Phe993 is responsible for the kdr-type resistance in German cockroach.
    • Molecular Sequence:
      GENBANK U73583; U73584
    • Accession Number:
      0 (DNA, Complementary)
      0 (Insect Proteins)
      0 (Pyrethrins)
      0 (Sodium Channels)
      47E5O17Y3R (Phenylalanine)
      GMW67QNF9C (Leucine)
    • Publication Date:
      Date Created: 19970201 Date Completed: 19970411 Latest Revision: 20191024
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/s0965-1748(96)00082-3
    • Accession Number:
      9066120