IDENTIFICACIÓN DE LOS PRINCIPALES COMPONENTES DE LA RAÍZ DE WEREQUE, EXTRAÍBLES EN MEDIO ACUOSO, POR GC-MS. (Spanish)

IDENTIFICATION OF THE MAIN WEREQUE'S ROOT COMPONENTS, EXTRACTIBLE IN AQUEOUS MEDIUM, BY GC-MS. (English)

This work comprises the identification of the Wereque's root main components. For that purpose, aqueous extractions of Wereque's fresh roots were made by infusion, decoction (both neutral and basic), and maceration, from which six samples were obtained for analysis. The raw extracts were filtered, purified and derivatized before they were analyzed. The GC-MS analyses were conducted on a Perkin Elmer Clarus 600T instrument, an Elite-5 MS, 30 m capillar column, ϕint: 0.32 mm, film: 0.25 μm; He: 0.8 mL/min; Tinj: 200 °C; Tsource and Ttransfer line : 230 °C; Tcolumn: 60 °C, 5min; 60 a 180°C, 20°C/min; 180°C, 10min; 70 eV, m/z 30 a 450. Through this work, 33 different compounds were identified, including palmitic acid. Based on existing literature, this component belongs to a group of fatty acids and monoglycerides responsible of the Wereque's hypoglycemic activity. [ABSTRACT FROM AUTHOR]

En este trabajo se realizó la identificación de los principales componentes de la raíz de Wereque por GC-MS. A partir de extracciones acuosas de las raíces frescas de Wereque mediante infusión, decocción neutra y básica, y maceración, se obtuvieron seis muestras a analizar. Los extractos crudos fueron filtrados, purificados y derivatizados antes de ser analizados. Los análisis por GC-MS fueron realizados en un equipo Perkin Elmer Clarus 600T, con una columna capilar Elite-5 MS 30 m, ϕint: 0.32 mm, film: 0.25 μm; He: 0.8 mL/min, Tiny: 200 °C; Tfuente y Tlínea de transferencia: 230 °C; Tcolumna: 60 °C, 5min; 60 a 180°C, 20°C/min; 180°C, 10min; 70 eV, m/z 30 a 450. Se identificaron 33 compuestos diferentes, entre los cuales se encuentra el ácido palmítico. Este compuesto forma parte de un grupo de ácidos grasos y monoglicéridos a los que se les atribuye la actividad hipoglucemiante del Wereque. [ABSTRACT FROM AUTHOR]

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