Definition of the mitogenic factor (MF) as a novel streptococcal superantigen that is different from streptococcal pyrogenic exotoxins A, B, and C.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 1273201 Publication Model: Print Cited Medium: Print ISSN: 0014-2980 (Print) Linking ISSN: 00142980 NLM ISO Abbreviation: Eur J Immunol Subsets: MEDLINE
    • Publication Information:
      Publication: <2005->: Weinheim : Wiley-VCH
      Original Publication: Weinheim, Verlag Chemie GmbH.
    • Subject Terms:
      Membrane Proteins*; Bacterial Proteins/*chemistry ; Exotoxins/*chemistry ; Mitogens/*chemistry ; Pyrogens/*chemistry ; Streptococcus pyogenes/*immunology ; Superantigens/*chemistry; Bacterial Proteins/genetics ; Bacterial Proteins/pharmacology ; Exotoxins/immunology ; Exotoxins/pharmacology ; HLA-DR Antigens/biosynthesis ; Humans ; Lymphocyte Activation/drug effects ; Mitogens/genetics ; Mitogens/pharmacology ; Pyrogens/immunology ; Pyrogens/pharmacology ; Receptors, Antigen, T-Cell, alpha-beta/biosynthesis ; Receptors, Antigen, T-Cell, alpha-beta/genetics ; Superantigens/pharmacology
    • Abstract:
      Human T cell activation by recombinant mitogenic factor (rMF) was investigated in comparison with that by recombinant streptococcal pyrogenic exotoxins (rSPE) A, B, and C. Recombinant MF, rSPEA, and rSPEC were mitogenic for peripheral blood mononuclear cells (PBMC), whereas rSPEB was not. Recombinant MF required only HLA-DR for the stimulation of PBMC, as determined using monoclonal antibodies (mAb) to HLA class II molecules and the mouse L cells transfected with HLA class II molecules. Recombinant SPEA and rSPEC required HLA-DR or HLA-DQ molecule. Recombinant MF selectively stimulated V beta 2, V beta 7, V beta 8, V beta 18 and V beta 21-bearing T cells, whereas rSPEA and rSPEC activated V beta 2 and V beta 6-bearing T cells as evaluated by the quantitative T cell receptor (TCR) analytical method. No clonality was observed in the nucleotide sequences of complementarity determining region 3 of TCR V beta in T cells responding to rMF. The profiles of cytokine production by PBMC in response to rMF, rSPEA, and rSPEC were quite similar. In summary, these results demonstrate that both HLA class II molecules and the TCR V beta required for rMF-mediated T cell activation are distinct from those required for rSPEA or rSPEC-mediated activation. Therefore, the MF is a novel streptococcal super-antigen which is different from SPEA, SPEB, and SPEC.
    • Accession Number:
      0 (Bacterial Proteins)
      0 (Exotoxins)
      0 (HLA-DR Antigens)
      0 (Membrane Proteins)
      0 (Mitogens)
      0 (Pyrogens)
      0 (Receptors, Antigen, T-Cell, alpha-beta)
      0 (SpeA protein, Streptococcus pyogenes)
      0 (Superantigens)
      0 (erythrogenic toxin)
      0 (mitogenic factor, Streptococcus)
    • Publication Date:
      Date Created: 19961101 Date Completed: 19970108 Latest Revision: 20041203
    • Publication Date:
      20231215
    • Accession Number:
      10.1002/eji.1830261122
    • Accession Number:
      8921957