New variants of enzyme immunoassay of antibodies to DNA.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print Cited Medium: Print ISSN: 0003-2700 (Print) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Chemical Society.
    • Subject Terms:
      Antibodies, Antinuclear/*analysis ; DNA/*immunology; Animals ; Immunoenzyme Techniques ; Mink ; Spectrophotometry
    • Abstract:
      A method of DNA immobilization on cellulose nitrate films has been developed. Modified films of uniform and stable surface have been used to devise two variants of solid-phase enzyme immunoassays of antibodies. The co-immobilization of enzyme label (cholinesterase) and the DNA molecules makes it possible to carry out the procedure of solid-phase enzyme immunoassay without any separation of components. Thus, it takes only 15 min to diagnose an autoimmune disease (Aleutian disease of minks) with the immunoenzyme amperometric sensor, with a lower detection limit for antibodies of 0.5 x 10(-10) M. For scaled diagnosing, solid-phase enzyme immunoassay on DNA-modified films with prior separation of components and spectrophotometric registration of peroxidase activity has been developed. The time for determination was 30 min, with a lower detection limit of 7.4 x 10(-12) M.
    • Accession Number:
      0 (Antibodies, Antinuclear)
      9007-49-2 (DNA)
    • Publication Date:
      Date Created: 19961101 Date Completed: 19961212 Latest Revision: 20190717
    • Publication Date:
      20221213
    • Accession Number:
      10.1021/ac950645v
    • Accession Number:
      8914484