Geminivirus Transmitidos por Mosca Blanca (Bemisia tabaci) en Tomate, en el Valle Agrícola de Culiacán, Sinaloa.

Geminivirus Transmitted by White Fly (Bemisia tabaci) in Tomato, in the Agricultural Valley of Culiacan, Sinaloa.

TRANSMISSION of virus diseases of plants; SWEETPOTATO whitefly; POLYMERASE chain reaction; NUCLEOTIDE sequence

SINALOA (Mexico : State); CULIACAN River Valley (Mexico)

Sinaloa es uno de los principales productores de tomate en México, sin embargo, su producción se ha visto afectada por enfermedades relacionadas con la presencia de geminivirus transmitidos por mosca blanca, ocasionando fuertes pérdidas económicas. En el presente trabajo, se identificaron y caracterizaron los geminivirus presentes en plantas de tomate, mediante la técnica de reacción en cadena de la polimerasa (PCR) anidada y posterior análisis de secuenciación. De 507 muestras de plantas analizadas, 197 presentaron síntomas y 310 no los mostraron; se encontraron un total de 134 muestras positivas, que corresponden a 99 (73.8%) que presentaron síntomas y 35 (26.2%) que no los presentaron. De las 134 muestras positivas detectadas por PCR anidada, 64.2% presentó el virus TYLCV, 22.4% SLCV, 6.7% correspondieron a variantes de otros virus (STLCV AV1, PHYVV, CuLCrV segmento A y B) y 3.7% no se identificó el tipo de geminivirus. La PCR anidada permitió detectar los geminivirus transmitidos por mosca presentes en plantas de tomate, tanto con síntomas como sin ellos, y su posterior secuenciación permitió determinar los diferentes tipos de geminivirus presentes, así como nuevas variantes de geminivirus y virus que no habían sido reportados previamente en tomate. [ABSTRACT FROM AUTHOR]

Sinaloa is one of the main tomato producer states in Mexico; however, its tomato production has been seriously affected by diseases related to the presence of geminivirus transmitted by whitefly, causing great economic losses for the state. In this study, geminivirus present in tomato plants were identified and characterized by nested polymerase chain reaction (PCR) and later sequencing analysis. A total of 507 plants were analyzed, from which, 197 plants showed symptoms of the presence of geminivirus and 310 plants did not show any symptoms of the presence of geminivirus. After analysis, 134 samples were found positive, 99 (73.8%) corresponding to plants that presented symptoms, and 35 (26.1%) without symptoms. From the 134 positives samples detected by nested PCR, 64.2% belonged to TYLCV, 22.4% to SLCV, 6.7% were variants of other viruses (STLCV AV1, PHYVV, CuLCrV segment A and B) and 3.7% contained geminivirus whose type was not identified. The nested PCR allowed detection of geminivirus transmitted by whitefly in tomato plants with, and without symptoms. Posterior sequencing analysis allowed to determine the different types of virus, as well as, new variants of geminivirus which has not been previously detected in tomato plants. [ABSTRACT FROM AUTHOR]

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