Development of a microchamber which spontaneously selects high-quality sperm for use in in vitro fertilization or micromanipulation.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Lih CH;Lih CH; Obasaju M; McCaffrey C; Gordon JW
  • Source:
    Journal of assisted reproduction and genetics [J Assist Reprod Genet] 1996 Sep; Vol. 13 (8), pp. 657-62.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer Country of Publication: Netherlands NLM ID: 9206495 Publication Model: Print Cited Medium: Print ISSN: 1058-0468 (Print) Linking ISSN: 10580468 NLM ISO Abbreviation: J Assist Reprod Genet Subsets: MEDLINE
    • Publication Information:
      Publication: <2004- >: Amsterdam : Springer
      Original Publication: New York : Plenum Press, c1992-
    • Subject Terms:
      Spermatozoa*/physiology; Cell Separation/*instrumentation ; Fertilization in Vitro/*instrumentation ; Micromanipulation/*instrumentation; Acrosome/physiology ; Humans ; Male ; Oligospermia/pathology ; Sperm Motility
    • Abstract:
      Background: A microchamber has been developed which allows motile sperm to swim from a central loading site to peripheral sidewells. The sidewells are designed such that oocytes may be placed within them for in vitro fertilization (IVF) or sperm may be harvested from them for use in standard IVF or micromanipulation. Because only motile sperm can reach the sidewells, the microchamber can select relatively high-quality sperm from a crude preparation. Moreover the steep walls of the sidewells create the potential to trap sperm.
      Objective: The present study was under taken to compare sperm in the sidewells to those found in randomly sampled regions of microchamber after various periods of incubation.
      Results: We find that the sidewells concentrate motile sperm and that a higher percentage of sperm removed from sidewells is acrosome reacted. Motile sperm from oligospermic patients can be harvested from microchamber sidewells for use in micromanipulation after loading the microchamber with unprocessed specimens. Results suggest that this microchamber could be used to enhance sperm:egg interaction in IVF or to harvest sperm for micromanipulation.
    • References:
      Fertil Steril. 1984 Apr;41(4):603-8. (PMID: 6705937)
      Biol Reprod. 1976 Nov;15(4):471-6. (PMID: 974200)
      J Androl. 1988 Nov-Dec;9(6):367-76. (PMID: 3215823)
      J Urol. 1992 Jun;147(6):1539-41. (PMID: 1593684)
      Fertil Steril. 1981 Jun;35(6):662-5. (PMID: 7250392)
      Fertil Steril. 1979 Mar;31(3):321-7. (PMID: 437167)
      Hum Reprod. 1993 Jul;8(7):1087-92. (PMID: 7691866)
      Fertil Steril. 1994 Sep;62(3):639-41. (PMID: 8062963)
      J Histochem Cytochem. 1989 Aug;37(8):1291-2. (PMID: 2474023)
      Fertil Steril. 1981 Aug;36(2):219-21. (PMID: 7262337)
      Fertil Steril. 1985 Feb;43(2):268-73. (PMID: 3967785)
      Fertil Steril. 1995 Aug;64(2):437-40. (PMID: 7615126)
    • Publication Date:
      Date Created: 19960901 Date Completed: 19970311 Latest Revision: 20190515
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/BF02069645
    • Accession Number:
      8897126