Detection of hydroxyl and carbon-centred radicals by EPR spectroscopy after ischaemia and reperfusion of the rat kidney.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Informa Healthcare Country of Publication: England NLM ID: 9423872 Publication Model: Print Cited Medium: Print ISSN: 1071-5762 (Print) Linking ISSN: 10292470 NLM ISO Abbreviation: Free Radic Res Subsets: MEDLINE
    • Publication Information:
      Publication: London : Informa Healthcare
      Original Publication: Yverdon, Switzerland : New York, NY : Harwood Academic ; distributed by STBS Ltd., c1994-
    • Subject Terms:
      Reperfusion*; Carbon/*chemistry ; Ischemia/*metabolism ; Kidney/*blood supply ; Kidney/*metabolism; Animals ; Cyclic N-Oxides/pharmacology ; Electron Spin Resonance Spectroscopy/methods ; Free Radicals ; Hydroxyl Radical ; In Vitro Techniques ; Kidney/drug effects ; Leukocytes/metabolism ; Male ; Nitrogen Oxides/pharmacology ; Rats ; Rats, Wistar
    • Abstract:
      Recent studies suggest that oxygen-derived free radicals are involved in mediating renal reperfusion injury. EPR spectroscopy and spin trapping with the spin traps DMPO and PBN, were used to detect and quantitate the formation of hydroxyl radicals in rat kidney after ischaemia-reperfusion in vivo and in vitro in the isolated rat kidney perfused in the absence of leucocytes. EPR analysis of homogenised kidneys and of venous samples did not detect radical adducts with either spin trap. With PBN, radical adducts were not detected in vitro. When DMPO was used as the spin trap in kidneys perfused without albumin in the perfusate, EPR signals characteristic of hydroxyl and carbon-centred radical adducts were detected during early reperfusion following ischaemia. These studies confirm the generation of hydroxyl radicals during ischaemia-reperfusion in kidney. During reperfusion the total DMPO adduct concentration reached 4.35 +/- 1.05 nmol/g kidney/3 min, p < 0.05. In control kidneys total adduct were present at lower concentration (2.55 +/- 1.1 nmol/g kidney/3 min). Addition of 15 mM dimethylthiourea abolished formation of these adducts following ischaemia-reperfusion but did not prevent a reduction in glomerular filtration rate. These results indicate that significant levels of hydroxyl and carbon-centred radicals are formed in the absence of circulating neutrophils during early renal reperfusion following ischaemia.
    • Accession Number:
      0 (Cyclic N-Oxides)
      0 (Free Radicals)
      0 (Nitrogen Oxides)
      3352-57-6 (Hydroxyl Radical)
      3I91332OPG (phenyl-N-tert-butylnitrone)
      7170JZ1QF3 (5,5-dimethyl-1-pyrroline-1-oxide)
      7440-44-0 (Carbon)
    • Publication Date:
      Date Created: 19960701 Date Completed: 19961210 Latest Revision: 20190920
    • Publication Date:
      20240627
    • Accession Number:
      10.3109/10715769609145654
    • Accession Number:
      8814442