[Establishing human, conjunctival fibroblast cultures as a test system for evaluating ophthalmic drugs].

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  • Author(s): Frantz A;Frantz A; Becker J
  • Source:
    Klinische Monatsblatter fur Augenheilkunde [Klin Monbl Augenheilkd] 1996 Mar; Vol. 208 (3), pp. 181-7.
  • Publication Type:
    Comparative Study; English Abstract; Journal Article
  • Language:
    German
  • Additional Information
    • Transliterated Title:
      Etablierung humaner, konjunktivaler Fibroblastenkulturen als Testsystem zur Beurteilung von Ophthalmika.
    • Source:
      Publisher: Thieme Country of Publication: Germany NLM ID: 0014133 Publication Model: Print Cited Medium: Print ISSN: 0023-2165 (Print) Linking ISSN: 00232165 NLM ISO Abbreviation: Klin Monbl Augenheilkd Subsets: MEDLINE
    • Publication Information:
      Publication: Stuttgart : Thieme
      Original Publication: Stuttgart.
    • Subject Terms:
      Anti-Inflammatory Agents/*pharmacology ; Burns, Chemical/*drug therapy ; Conjunctiva/*drug effects ; Drug Evaluation, Preclinical/*methods ; Eye Burns/*chemically induced; Adult ; Aprotinin/pharmacology ; Burns, Chemical/pathology ; Cell Division/drug effects ; Cells, Cultured ; Chloramphenicol/pharmacology ; Conjunctiva/pathology ; Culture Media ; Eye Burns/drug therapy ; Female ; Fibroblasts/drug effects ; Fibroblasts/pathology ; Humans ; Hypromellose Derivatives ; Male ; Methylcellulose/analogs & derivatives ; Methylcellulose/pharmacology ; Middle Aged ; Ophthalmic Solutions ; Prednisolone/pharmacology ; Protease Inhibitors/pharmacology
    • Abstract:
      Background: Chronic inflammation of the anterior eye segment caused by severe burns has to be treated by several drugs. Often it is very difficult to recognize the relationship between drug and side effects. Although the results were usually satisfying, proliferation of the conjunctival tissue was observed. Therefore the question was raised whether these proliferations might have been a side effect of the drugs. To answer this question, we established cultures of human conjunctival fibroblasts for ocular toxicity testing of drugs used in the therapy of severe eye burns.
      Materials and Methods: Conjunctival fibroblasts from young donors were cultured under standard conditions (37 degrees C, 5% C02, 95% RH) in Medium 199 supplemented with 20% FCS without antibiotics. At the time of inoculation the following drugs were added: aprotinin, prednisolone, chloramphenicol and methylhydroxypropylcellulose. Cell growth was observed and growth kinetics were estimated by hemocytometer over a period of 7 days.
      Results: The investigations of prednisolone showed the well-known dose-dependent anti-proliferative effect. The application of methylhydroxypropylcellulose resulted in decreased cell growth and in total cell detachment. Experiments with aprotinin and chloramphenicol showed no effects on the growth behaviour. The application of a drug mixture lead to similar results as in experiments with prednisolone.
      Discussion: The presented cell-culture system is able to reproduce specific effects, especially the toxicity of ophthalmic drugs but a complex interaction of an inflammatory reaction, e.g. after severe eye burns, cannot be simulated. The network of reactions and the interaction of many substances playing an important role during this process are too complex.
    • Accession Number:
      0 (Anti-Inflammatory Agents)
      0 (Culture Media)
      0 (Ophthalmic Solutions)
      0 (Protease Inhibitors)
      3NXW29V3WO (Hypromellose Derivatives)
      66974FR9Q1 (Chloramphenicol)
      9004-67-5 (Methylcellulose)
      9087-70-1 (Aprotinin)
      9PHQ9Y1OLM (Prednisolone)
    • Publication Date:
      Date Created: 19960301 Date Completed: 19960905 Latest Revision: 20141120
    • Publication Date:
      20221213
    • Accession Number:
      10.1055/s-2008-1035193
    • Accession Number:
      8699776