Antigenic analysis by agglutination of Trypanosoma brucei brucei parasitemias initiated in mice with in vitro-produced metacyclics.

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  • Author(s): Stanley HA; Honigberg BM; Cunningham I
  • Source:
    Zeitschrift fur Parasitenkunde (Berlin, Germany) [Z Parasitenkd] 1979 Feb 28; Vol. 58 (2), pp. 141-9.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer Verlag Country of Publication: Germany NLM ID: 8710749 Publication Model: Print Cited Medium: Print ISSN: 0044-3255 (Print) Linking ISSN: 00443255 NLM ISO Abbreviation: Z Parasitenkd Subsets: MEDLINE
    • Publication Information:
      Publication: Berlin : Springer Verlag
      Original Publication: Berlin : Verlag von Julius Springer, 1934-c1986.
    • Subject Terms:
      Antigens/*analysis ; Trypanosoma brucei brucei/*immunology ; Trypanosomiasis, African/*immunology; Adsorption ; Agglutination Tests/methods ; Animals ; Cross Reactions ; Epitopes ; Female ; Immune Sera/isolation & purification ; Mice ; Rabbits ; Trypanosoma brucei brucei/pathogenicity
    • Abstract:
      Trypanosomes from 14 first-peak parasitemias initiated in mice by injection of in vitro-produced metacyclics were stabilated. Strains derived from these stabilates were analyzed for their antigenic composition by cross-agglutination with immune sera produced in rabbits against 12 of the stabilates. The antigenic composition of the 14 stabilates was compared also with two first-peak parasitemias from mice inoculated with fly-derived metacyclics, the variant-specific antigen of the strain used to initiate the cultures that ultimately became infective, and the antigenic variant that was used to infect the flies. One variant-specific, presumably basic, antigen was found, either as the predominant (nine parasitemias) or as a minor (seven parasitemias) antigen, in all first peak-parasitemia strain initiated with culture- or fly-derived metacyclics; it was absent, however, from the strains (not first-peak parasitemias) used to start the cultures or to infect the flies. Only one of the first-peak parasitemias appeared to have the basic antigen alone. The remaining parasitemia populations seemed to have from about two to six antigens, some of which were common to culture- and fly-derived infections. There was very little, if any, antigenic relationship between the foregoing populations and the strains employed for initiation of cultures or for infection of flies. It is evident from the results that much antigenic similarity exists between the culture- and tsetse fly-derived first-peak parasitemias.
    • References:
      Trans R Soc Trop Med Hyg. 1975;69(1):131-8. (PMID: 49958)
      Trans R Soc Trop Med Hyg. 1962 Jan;56:48-59. (PMID: 13882652)
      J Gen Microbiol. 1965 Nov;41(2):195-214. (PMID: 5866129)
      Exp Parasitol. 1970 Dec;28(3):521-34. (PMID: 4993889)
      Acta Trop. 1977 Mar;34(1):35-41. (PMID: 67786)
      Science. 1977 Sep 23;197(4310):1279-82. (PMID: 897667)
      Nature. 1978 May 25;273(5660):300-2. (PMID: 306579)
      Exp Parasitol. 1976 Jun;39(3):496-522. (PMID: 773657)
      Ann Soc Belg Med Trop. 1977;57(4-5):383-8. (PMID: 610617)
      Ann Soc Belg Med Trop. 1977;57(4-5):369-81. (PMID: 610616)
    • Accession Number:
      0 (Antigens)
      0 (Epitopes)
      0 (Immune Sera)
    • Publication Date:
      Date Created: 19790228 Date Completed: 19790629 Latest Revision: 20190911
    • Publication Date:
      20250114
    • Accession Number:
      10.1007/BF01951338
    • Accession Number:
      86245