Stimulatory effect of ouabain on VCAM-1 and iNOS expression in murine endothelial cells: involvement of NF-kappa B.

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  • Author(s): Bereta J;Bereta J; Cohen MC; Bereta M
  • Source:
    FEBS letters [FEBS Lett] 1995 Dec 11; Vol. 377 (1), pp. 21-5.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print Cited Medium: Print ISSN: 0014-5793 (Print) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE
    • Publication Information:
      Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd.
      Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies.
    • Subject Terms:
      Endothelium, Vascular/*metabolism ; Gene Expression/*drug effects ; NF-kappa B/*metabolism ; Nitric Oxide Synthase/*genetics ; Ouabain/*pharmacology ; Vascular Cell Adhesion Molecule-1/*genetics; Animals ; Base Sequence ; Blotting, Northern ; Brain/blood supply ; Humans ; Interferon-gamma/pharmacology ; Mice ; Molecular Sequence Data ; Nitric Oxide/metabolism ; Nitrites/metabolism ; RNA, Messenger/analysis ; Recombinant Proteins/pharmacology ; Tumor Necrosis Factor-alpha/pharmacology
    • Abstract:
      Endothelial cells play a pivotal role in the development of atherosclerosis. An 'activated' phenotype of these cells is manifested by signal transduction-dependent expression of genes encoding cytokines, pro- and anticoagulant factors, and cell adhesion molecules. In the current study we examined the effect of ouabain, an inhibitor of Na+/K(+)-ATPase, on the process of endothelial cell activation. We demonstrated that ouabain was able to stimulate VCAM-1 expression and potentiate the effect of IFN-gamma on this process. Moreover, ouabain provided a complementary signal for either TNF or IFN-gamma in inducing iNOS expression. Our data also show, for the first time, that inhibition of Na+/K(+)-ATPase led to activation of the transcription factor, NF-kappa B, which may provide an explanation for the effects of ouabain on endothelial cells.
    • Accession Number:
      0 (NF-kappa B)
      0 (Nitrites)
      0 (RNA, Messenger)
      0 (Recombinant Proteins)
      0 (Tumor Necrosis Factor-alpha)
      0 (Vascular Cell Adhesion Molecule-1)
      31C4KY9ESH (Nitric Oxide)
      5ACL011P69 (Ouabain)
      82115-62-6 (Interferon-gamma)
      EC 1.14.13.39 (Nitric Oxide Synthase)
    • Publication Date:
      Date Created: 19951211 Date Completed: 19960209 Latest Revision: 20190621
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/0014-5793(95)01301-6
    • Accession Number:
      8543010