Identity of a major 3-deoxyglucosone-reducing enzyme with aldehyde reductase in rat liver established by amino acid sequencing and cDNA expression.

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  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print Cited Medium: Print ISSN: 0378-1119 (Print) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
    • Subject Terms:
      Aldehyde Reductase/*genetics ; Aldehyde Reductase/*metabolism ; DNA/*genetics ; Deoxyglucose/*analogs & derivatives ; Liver/*enzymology; Amino Acid Sequence ; Animals ; Base Sequence ; Cell Line ; Cloning, Molecular ; DNA/isolation & purification ; Deoxyglucose/metabolism ; Genetic Vectors ; Humans ; Kinetics ; Molecular Sequence Data ; Polymerase Chain Reaction ; Rats ; Recombinant Proteins/metabolism ; Substrate Specificity ; Transfection
    • Abstract:
      We have purified a rat liver enzyme that catalyzes the NADPH-dependent reduction of 3-deoxyglucosone (3-DG), a major intermediate in the Maillard reaction and a potent cross-linker responsible for the polymerization of proteins. Comparison of the amino acid (aa) sequences of nine peptides obtained from the rat 3-DG-reducing enzyme by lysylendopeptidase digestion with the aa sequence of human aldehyde reductase (ALR) [Bohren et al., J. Biol. Chem. 266 (1991) 24031-24037] strongly suggested that the purified enzyme was rat ALR. We cloned the cDNA encoding ALR from a rat kidney cDNA library using a human ALR cDNA fragment, amplified by polymerase chain reaction, as a probe. All nine peptides identified in the purified rat 3-DG-reducing enzyme were found in the aa sequence deduced from the rat ALR cDNA. Moreover, cell extract from COS-1 cells transfected with the rat ALR cDNA exhibited NADPH-dependent 3-DG-reducing activity and cross-reacted with antiserum raised against the purified rat 3-DG-reducing enzyme. All the above data indicate clearly that the 3-DG-reducing enzyme is identical with ALR. Northern blot analysis of total mRNA from a variety of rat tissues showed fairly high levels of expression of ALR mRNA. This suggests that sufficient ALR is present to detoxify 3-DG when it is formed through the Maillard reaction in vivo.
    • Gene Symbol:
      ALR
    • Molecular Sequence:
      GENBANK D10854; L03653; L03654; L07632; L09602; X54209; X67280; X67281; X67282; X67283
    • Accession Number:
      0 (Recombinant Proteins)
      2490-91-7 (3-deoxyglucose)
      9007-49-2 (DNA)
      9G2MP84A8W (Deoxyglucose)
      EC 1.1.1.21 (Aldehyde Reductase)
    • Publication Date:
      Date Created: 19930530 Date Completed: 19930629 Latest Revision: 20190707
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/0378-1119(93)90728-l
    • Accession Number:
      8500767