A Rab32-Dependent Pathway Contributes to Salmonella Typhi Host Restriction.

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    • Subject Terms:
      SALMONELLA typhi; HOST-parasite relationships; MACROPHAGES; SALMONELLA typhimurium; BIOLOGICAL transport; SALMONELLA; RNA
    • Abstract:
      Unlike other Salmonellae, the intracellular bacterial human pathogen Salmonella Typhi exhibits strict host specificity. The molecular bases for this restriction are unknown. Here we found that the expression of a single type III secretion system effector protein from broad-host Salmonella Typhimurium allowed Salmonella Typhi to survive and replicate within macrophages and tissues from mice, a nonpermissive host. This effector proteolytically targeted Rab32, which controls traffic to lysosome-related organelles in conjunction with components of the biogenesis of lysosome-related organelle complexes (BLOCs). RNA interference-mediated depletion of Rab32 or of an essential component of a BLOC complex was sufficient to alLow S. Typhi to survive within mouse macrophages. Furthermore, S. Typhi was able to survive in macrophages from mice defective in BLOC components. [ABSTRACT FROM AUTHOR]
    • Abstract:
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