Endocytosis of alpha 1-acid glycoprotein variants by human monocytic lineage cells.

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  • Additional Information
    • Source:
      Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 8108529 Publication Model: Print Cited Medium: Print ISSN: 0248-4900 (Print) Linking ISSN: 02484900 NLM ISO Abbreviation: Biol Cell Subsets: MEDLINE
    • Publication Information:
      Publication: 2012- : Chichester : Wiley-Blackwell
      Original Publication: Ivry sur Seine France : Publié par la Société française de microscopie électronique avec le concours du Centre national de la recherche scientifique et de l'Institute national de la santé et de la recherche médicale, [1981-
    • Subject Terms:
      Endocytosis/*physiology ; Monocytes/*immunology ; Orosomucoid/*chemistry; Cell Line ; Cellular Senescence/physiology ; Flow Cytometry ; Glycoproteins/metabolism ; Humans ; Macrophages/immunology ; Microscopy/methods ; Orosomucoid/metabolism ; Protein Binding
    • Abstract:
      Human alpha 1-acid glycoprotein (AGP or orosomucoid) is a major glycoprotein of plasma. AGP can be separated on immobilized concanavalin A into three variants bearing none (AGP A), one (AGP B) or two (AGP C) biantennary glycans. In this paper, we show, using flow cytometry and confocal microscopy, that AGP C which is eluted from concanavalin A with mannose, binds to human monocytes, monocyte-derived macrophages as well as human promonocytic cell lines such as THP1 or U937. Conversely HL60, a promyelocytic cell line, does not express the surface AGP C binding protein. AGP C is internalized and degraded with an efficiency depending on the state of differentiation of these cells. In contrast, AGP A which is not recognized by concanavalin A, does not bind to any of these cells.
    • Accession Number:
      0 (Glycoproteins)
      0 (Orosomucoid)
    • Publication Date:
      Date Created: 19930101 Date Completed: 19931007 Latest Revision: 20191023
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/s0248-4900(05)80187-x
    • Accession Number:
      8364398