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Effects of added albumin on the kinetic isotope effect observed in the microsomal oxidative demethylation of the methoxyl group.
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- Author(s): Kurihara N;Kurihara N
- Source:
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1993 Aug 31; Vol. 195 (1), pp. 47-52.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
- Publication Information: Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press. - Subject Terms: Methoxychlor/*metabolism ; Microsomes, Liver/*metabolism ; Serum Albumin, Bovine/*pharmacology; Animals ; Biotransformation ; Cytochrome P-450 Enzyme System/metabolism ; Deuterium ; Gas Chromatography-Mass Spectrometry ; Kinetics ; Male ; Mathematics ; Microsomes, Liver/drug effects ; Radioisotope Dilution Technique ; Rats ; Rats, Wistar
- Abstract: The kinetic deuterium isotope effect on the microsomal oxidative demethylation of methoxyl groups was measured using methoxychlor, an insecticide, as a model compound. When the metabolic reactions were conducted in the presence of bovine serum albumin, the observed isotope effect was smaller than that in its absence. The values were unaltered when the albumin concentration was changed from 1% to 2%. The results were interpreted by the alteration of the ES-complex formation/dissociation rate with the interaction between substrate and albumin.
- Comments: Erratum in: Biochem Biophys Res Commun 1993 Nov 30;197(1):350.
- Accession Number: 27432CM55Q (Serum Albumin, Bovine)
9035-51-2 (Cytochrome P-450 Enzyme System)
AR09D82C7G (Deuterium)
RIA79UD69L (Methoxychlor) - Publication Date: Date Created: 19930831 Date Completed: 19930928 Latest Revision: 20171116
- Publication Date: 20221213
- Accession Number: 10.1006/bbrc.1993.2007
- Accession Number: 8363623
- Source:
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