Assessment of the influence of subacute phenobarbitone administration on multi-tissue cell proliferation in the rat using bromodeoxyuridine immunocytochemistry.

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  • Author(s): Jones HB;Jones HB; Clarke NA
  • Source:
    Archives of toxicology [Arch Toxicol] 1993; Vol. 67 (9), pp. 622-8.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0417615 Publication Model: Print Cited Medium: Print ISSN: 0340-5761 (Print) Linking ISSN: 03405761 NLM ISO Abbreviation: Arch Toxicol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin, New York, Springer-Verlag.
    • Subject Terms:
      Bromodeoxyuridine*; Phenobarbital/*toxicity; Adrenal Glands/drug effects ; Animals ; Cell Division/drug effects ; Immunohistochemistry ; Kidney/drug effects ; Liver/drug effects ; Male ; Pancreas/drug effects ; Phenobarbital/pharmacology ; Pituitary Gland/drug effects ; Rats ; Rats, Wistar ; Testis/drug effects ; Thyroid Gland/drug effects
    • Abstract:
      The effects of daily administration of phenobarbitone on the mitotic rates of several tissues were investigated by bromodeoxyuridine (BrdU) immunocytochemistry. Phenobarbitone (80 mg/kg per day) was dosed to AP Wistar male rats for up to 7 days and BrdU (10 mg/ml) was given by infusion at a rate of 10 microliters/h via subcutaneously implanted osmotic minipumps for 2 days prior to necropsy on days 1, 2, 3, 5 and 7. BrdU-labelled nuclei were visualised by peroxidase-antiperoxidase immunocytochemistry and counts of the numbers of labelled cells (labelling index, LI%) made from at least 1000 cells per tissue section(s). The LIs of several tissues (testis, adrenal cortex and medulla, kidney distal convoluted tubule and exocrine pancreas) showed no statistical difference by comparison with controls. Several tissues exhibited characteristic responses to phenobarbitone administration. Pituitary and endocrine pancreas LIs were decreased while those of thyroid, liver and kidney proximal convoluted tubule were increased. The pattern of LI increase was unique to each tissue with liver (median and lateral lobes) increased two-fold on day 3 and returning to control levels thereafter while kidney proximal tubule LI rose gradually with time and remained elevated on day 7. Thyroid LI on day 1 was almost double that of day 0 control and increased steadily thereafter. These data illustrate the varied responses of different tissues to phenobarbitone exposure, namely, depression and stimulation of mitosis. The causation of these functional changes is discussed in relation to direct and indirect effects on functional parameters, especially enzyme induction, alterations in hormonal and growth factor status and receptor regulation.
    • References:
      Endocrinology. 1985 Mar;116(3):873-8. (PMID: 2578951)
      Toxicol Appl Pharmacol. 1989 Jun 15;99(2):216-28. (PMID: 2734788)
      Science. 1990 Aug 31;249(4972):1007-11. (PMID: 2204108)
      Science. 1990 Aug 31;249(4972):970-1. (PMID: 2136249)
      Toxicol Appl Pharmacol. 1991 Mar 1;107(3):562-7. (PMID: 2000642)
      Toxicol Appl Pharmacol. 1991 Nov;111(2):263-78. (PMID: 1957312)
      Biochem J. 1991 Jul 15;277 ( Pt 2):577-80. (PMID: 1859384)
      Prog Clin Biol Res. 1990;331:345-65. (PMID: 2156272)
      Toxicol Appl Pharmacol. 1988 Jun 30;94(2):254-65. (PMID: 3388422)
      Carcinogenesis. 1987 Sep;8(9):1355-7. (PMID: 3621474)
      Proc Natl Acad Sci U S A. 1975 Mar;72(3):1157-60. (PMID: 1055372)
      Toxicol Pathol. 1989;17(2):294-306. (PMID: 2675280)
      J Histochem Cytochem. 1993 Jan;41(1):21-7. (PMID: 8093255)
      Acta Endocrinol (Copenh). 1977 May;85(1):189-197. (PMID: 577076)
      J Histochem Cytochem. 1991 Jan;39(1):23-30. (PMID: 1670579)
      Neuropathol Appl Neurobiol. 1990 Apr;16(2):123-33. (PMID: 2161084)
      Cancer Res. 1988 Dec 1;48(23):6739-44. (PMID: 3180084)
      Prog Clin Biol Res. 1991;369:457-67. (PMID: 1946540)
      Arch Biochem Biophys. 1988 May 15;263(1):204-15. (PMID: 3130800)
      Biochem Biophys Res Commun. 1991 Jan 15;174(1):331-7. (PMID: 1846541)
      Clin Pharmacol Ther. 1971 Jul-Aug;12(4):698-703. (PMID: 4998600)
      Proc Natl Acad Sci U S A. 1990 Oct;87(19):7772-6. (PMID: 2217209)
      Toxicol Appl Pharmacol. 1991 Sep 15;110(3):505-13. (PMID: 1949017)
      Adv Enzyme Regul. 1975;13:281-93. (PMID: 1211288)
      J Pharmacol Exp Ther. 1974 Feb;188(2):287-92. (PMID: 4811296)
      Regul Toxicol Pharmacol. 1989 Jun;9(3):284-95. (PMID: 2756175)
      Klin Wochenschr. 1989 Oct 2;67(19):976-9. (PMID: 2693820)
      J Histochem Cytochem. 1991 Aug;39(8):1125-30. (PMID: 1856460)
      Dig Dis Sci. 1991 May;36(5):659-68. (PMID: 2022168)
      Prog Clin Biol Res. 1990;331:149-61. (PMID: 2179957)
    • Accession Number:
      G34N38R2N1 (Bromodeoxyuridine)
      YQE403BP4D (Phenobarbital)
    • Publication Date:
      Date Created: 19930101 Date Completed: 19940314 Latest Revision: 20190904
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/BF01974069
    • Accession Number:
      8311689