Reversal of CAMAL-mediated alterations of normal and leukemic in vitro myelopoiesis using inhibitors of proteolytic activity.

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  • Author(s): Leitch HA;Leitch HA; Levy JG
  • Source:
    Leukemia [Leukemia] 1994 Apr; Vol. 8 (4), pp. 605-11.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Nature Publishing Group, Specialist Journals Country of Publication: England NLM ID: 8704895 Publication Model: Print Cited Medium: Print ISSN: 0887-6924 (Print) Linking ISSN: 08876924 NLM ISO Abbreviation: Leukemia Subsets: MEDLINE
    • Publication Information:
      Publication: 2000- : London : Nature Publishing Group, Specialist Journals
      Original Publication: [Baltimore, Md.] : Williams & Wilkins, [c1987-
    • Subject Terms:
      Carrier Proteins*; Amino Acid Chloromethyl Ketones/*pharmacology ; Antigens, Differentiation, Myelomonocytic/*pharmacology ; Biomarkers, Tumor/*pharmacology ; Phenylmethylsulfonyl Fluoride/*pharmacology ; Stem Cells/*drug effects; Antimicrobial Cationic Peptides ; Blood Proteins/pharmacology ; Cathepsin G ; Cathepsins/pharmacology ; Colony-Forming Units Assay ; Humans ; Serine Endopeptidases
    • Abstract:
      CAMAL (common antigen of myelogenous acute leukemia) is an antigenic preparation isolated in this laboratory from the bone marrow or peripheral blood leucocytes of persons with myeloid leukemias. Material from CAMAL preparations, which migrates in the range of 30 to 35 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, P30-35 CAMAL), was shown to exert an inhibitory effect on in vitro colony formation by progenitor cells from normal healthy donors. The same preparations of P30-35 CAMAL, in contrast, exerted a stimulatory effect on in vitro colony formation by progenitor cells from patients with chronic myelogenous leukemia (CML). We now report that both the inhibitory effect on normal colony formation and the stimulatory effect on CML colony formation mediated by P30-35 CAMAL were blocked using phenyl methyl sulfonyl fluoride (PMSF), an inhibitor of the activity of serine proteases. Similarly, both the P30-35 CAMAL-mediated inhibitory effect on normal colony formation and the P30-35 CAMAL-mediated stimulatory effect on CML colony formation were blocked using the peptide ala-pro-phe-CMK, also an inhibitor of serine protease activity. These results suggest the involvement of proteolytic activity, either directly or indirectly, in the alterations of in vitro myelopoiesis exerted by P30-35 CAMAL.
    • Accession Number:
      0 (AZU1 protein, human)
      0 (Amino Acid Chloromethyl Ketones)
      0 (Antigens, Differentiation, Myelomonocytic)
      0 (Antimicrobial Cationic Peptides)
      0 (Biomarkers, Tumor)
      0 (Blood Proteins)
      0 (Carrier Proteins)
      0 (alanyl-prolyl-phenylalanine chloromethyl ketone)
      0 (common myelogenous leukemia-associated antigen)
      57KD15003I (Phenylmethylsulfonyl Fluoride)
      EC 3.4.- (Cathepsins)
      EC 3.4.21.- (Serine Endopeptidases)
      EC 3.4.21.20 (CTSG protein, human)
      EC 3.4.21.20 (Cathepsin G)
    • Publication Date:
      Date Created: 19940401 Date Completed: 19940510 Latest Revision: 20210510
    • Publication Date:
      20250114
    • Accession Number:
      8152256