Monoclonal antibody to CD4+ T cells abrogates genetic resistance to Haemonchus contortus in sheep.

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  • Author(s): Gill HS;Gill HS; Watson DL; Brandon MR
  • Source:
    Immunology [Immunology] 1993 Jan; Vol. 78 (1), pp. 43-9.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 0374672 Publication Model: Print Cited Medium: Print ISSN: 0019-2805 (Print) Linking ISSN: 00192805 NLM ISO Abbreviation: Immunology Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford : Blackwell Scientific Publications
    • Subject Terms:
      CD4-Positive T-Lymphocytes/*immunology ; Haemonchiasis/*immunology ; Haemonchiasis/*veterinary ; Sheep Diseases/*immunology; Animals ; Antibodies, Monoclonal/immunology ; CD4 Antigens/immunology ; CD8 Antigens/immunology ; Haemonchiasis/parasitology ; Hematocrit ; Immunity, Innate/genetics ; Intestinal Mucosa/immunology ; Sheep
    • Abstract:
      The roles of CD4+ and CD8+ T cells in genetically determined resistance of sheep to Haemonchus contortus (a natural host-parasite relationship) was investigated by selectively depleting genetically resistant merino lambs of their CD4+ or CD8+ T cells by treatment with mouse monoclonal antibody (mAb) specific for the appropriate determinant before and during challenge infection. Administration of anti-CD4 mAb to genetically resistant lambs completely abrogated their expression of genetic resistance as indicated by significantly higher faecal egg output and worm burdens found in the CD4+ T-cell-depleted lambs compared with those of controls. Host responses associated with resistance to H. contortus including mucosal mast cell hyperplasia and tissue eosinophilia were also significantly suppressed in CD4-depleted lambs. The development of anamnestic anti-parasite antibody responses were also significantly inhibited by anti-CD4 mAb. Furthermore, anti-CD4 mAb abolished differences in host responses between genetically resistant and random-bred (susceptible) lambs. In contrast, depletion of CD8+ T cells had no effect on genetic resistance; faecal egg output, worm counts, mast cells and eosinophil responses in CD8-depleted lambs were not significantly different from those in controls. Together, these results suggest that CD4+ T cells play a pivotal role in mediating genetic resistance to H. contortus, and in the generation of mucosal mast cell hyperplasia, tissue eosinophilia and anti-Haemonchus antibody. CD8+ T cells appear to play no protective role. The possible mechanisms by which CD4+ T cells might mediate anti-parasite resistance are discussed.
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    • Accession Number:
      0 (Antibodies, Monoclonal)
      0 (CD4 Antigens)
      0 (CD8 Antigens)
    • Publication Date:
      Date Created: 19930101 Date Completed: 19930324 Latest Revision: 20181113
    • Publication Date:
      20240829
    • Accession Number:
      PMC1421769
    • Accession Number:
      8094709