The chemiluminescence assay of lipid peroxidation products in human blood plasma lipoproteins.

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  • Additional Information
    • Source:
      Publisher: Informa Healthcare Country of Publication: England NLM ID: 9423872 Publication Model: Print Cited Medium: Print ISSN: 1071-5762 (Print) Linking ISSN: 10292470 NLM ISO Abbreviation: Free Radic Res Subsets: MEDLINE
    • Publication Information:
      Publication: London : Informa Healthcare
      Original Publication: Yverdon, Switzerland : New York, NY : Harwood Academic ; distributed by STBS Ltd., c1994-
    • Subject Terms:
      Lipid Peroxidation* ; Luminescent Measurements*; Lipoproteins/*blood; Coloring Agents ; Coumarins ; Ethanol ; Humans ; Kinetics ; Lipoproteins, LDL/blood ; Spectrophotometry ; Thiobarbituric Acid Reactive Substances/analysis
    • Abstract:
      A chemiluminescence (CL) flash kinetics on the addition of Fe2+ ions into oxidized low density lipoprotein (LDL) suspension has been studied. LDL oxidation was carried out at 37 degrees C without and in the presence of 5 or 50 microM of Cu2+. It has been found that under certain experimental conditions (the addition of excess iron ions, more than 1 mM) the amplitude of CL flash depended almost linearly (1) on the concentration of oxidized LDL and (2) on the extent of LDL oxidation measured as diene conjugates (DC) and 2-thiobarbituric acid-reactive substance (TBARS) accumulation. The corresponding correlation coefficients were: for TBARS -0.94 and for DC -0.97, in the case of LDL autooxidation; 0.72 and 0.98, in the case of copper-induced LDL oxidation. A sensitivity of the CL method was shown to be significantly enhanced (by more than two orders) in the presence of CL sensitizer--2,3,5,6-1H,4H-tetrahydro-9-(2'-benzoimidazolyl)-qui nolizin-(9,9a,1- gh)coumarin.
    • Accession Number:
      0 (Coloring Agents)
      0 (Coumarins)
      0 (Lipoproteins)
      0 (Lipoproteins, LDL)
      0 (Thiobarbituric Acid Reactive Substances)
      3K9958V90M (Ethanol)
      A4VZ22K1WT (coumarin)
    • Publication Date:
      Date Created: 19940501 Date Completed: 19940923 Latest Revision: 20190920
    • Publication Date:
      20240829
    • Accession Number:
      10.3109/10715769409145627
    • Accession Number:
      8069385