PCR protocol- and inulin catabolism-based differentiation of inulinolytic soil bacteria.

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  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 8208561 Publication Model: Print Cited Medium: Print ISSN: 0273-2289 (Print) Linking ISSN: 02732289 NLM ISO Abbreviation: Appl Biochem Biotechnol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Clifton, N.J. : Humana Press, c1981-
    • Subject Terms:
      Polymerase Chain Reaction*; Bacteria/*isolation & purification ; Inulin/*metabolism; Arthrobacter/isolation & purification ; Bacteria/cytology ; Bacteria/genetics ; Base Sequence ; Culture Media ; DNA, Bacterial/analysis ; DNA, Bacterial/chemistry ; Disaccharides/analysis ; Disaccharides/metabolism ; Fructose/metabolism ; Molecular Sequence Data ; Polysaccharides, Bacterial/metabolism
    • Abstract:
      Bacteria collected from rotting dahlia tubers, instead of degrading inulin to D-fructose, preferentially formed the known DFA III (beta-2.1': alpha-2',3 difructofuranose anhydride), inulobiose, higher inulo-oligosaccharides, and exoheteropolysaccharides. Owing to the morphological and Gram staining variability, the bacterial isolates designated YLW and CRM were examined to differentiate them from a reference strain Arthrobacter ureafaciens. The comparative analyses were whole DNA random amplification by Taq polymerase (RAPD-PCR protocol), culture media DFA III content in culture media, chromatographic profile of oligosaccharides formed, and exopolysaccharide fractionation/fragmentation. A comparative study in liquid shake cultures showed that the isolate YLW was faster than the reference strain in the production of DFA III when the inulin/yeast extract ratio was maintained at 10 in the medium, although a similar maximum yield was displayed with both bacteria (13-14 mg of DFA/mL cell free media from the initial 30 mg/mL of inulin load). Doubling the yeast extract input, an even faster onset of DFA III production occurred with YLW but with no further improvement in the maximum yield. Both strains further degraded the resulting DFA during the stationary growth phase. The main ability of CRM when grown on inulin was the production of exopolysaccharides, although culture condition variation also allowed DFA III production, which was accompanied by somewhat lower amounts of its reducing analog, inulobiose.
    • References:
      Nucleic Acids Res. 1991 Oct 11;19(19):5275-9. (PMID: 1923811)
      Nucleic Acids Res. 1990 Dec 25;18(24):7213-8. (PMID: 2259619)
      Theor Appl Genet. 1991 Jul;82(4):473-6. (PMID: 24213264)
      Biochim Biophys Acta. 1972 Sep 19;284(1):248-56. (PMID: 4561389)
    • Accession Number:
      0 (Culture Media)
      0 (DNA, Bacterial)
      0 (Disaccharides)
      0 (Polysaccharides, Bacterial)
      30237-26-4 (Fructose)
      81129-73-9 (difructose anhydride III)
      9005-80-5 (Inulin)
    • Publication Date:
      Date Created: 19940101 Date Completed: 19940715 Latest Revision: 20190909
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/BF02941805
    • Accession Number:
      8010763