Endotoxin stimulates interleukin-6 production in intestinal epithelial cells. A synergistic effect with prostaglandin E2.

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  • Additional Information
    • Source:
      Publisher: American Medical Association Country of Publication: United States NLM ID: 9716528 Publication Model: Print Cited Medium: Print ISSN: 0004-0010 (Print) Linking ISSN: 00040010 NLM ISO Abbreviation: Arch Surg Subsets: MEDLINE
    • Publication Information:
      Original Publication: Chicago : American Medical Association, c1960-
    • Subject Terms:
      Escherichia coli*; Dinoprostone/*immunology ; Interleukin-6/*metabolism ; Intestinal Mucosa/*metabolism ; Lipopolysaccharides/*immunology; Animals ; Cell Line ; Cells, Cultured ; Dinoprostone/pharmacology ; Dose-Response Relationship, Drug ; Drug Evaluation, Preclinical ; Drug Synergism ; Epithelium ; Indomethacin/immunology ; Indomethacin/pharmacology ; Interleukin-6/analysis ; Interleukin-6/immunology ; Intestinal Mucosa/immunology ; Polymerase Chain Reaction ; RNA, Messenger/analysis ; Rats
    • Abstract:
      Objective: To test the hypothesis that endotoxin stimulates the release of interleukin-6 (IL-6) from intestinal epithelial cells and that this effect of endotoxin is regulated by prostaglandin E2 (PGE2).
      Design: A rat intestinal crypt cell line, IEC-6, was cultured in the presence of lipopolysaccharide (LPS), 0.1 to 1.0 microgram/mL, and/or PGE2, 1 mumol/L. In other experiments, indomethacin, 20 mumol/L, was added to LPS-treated cells to block the effects of prostaglandins. Control wells contained medium alone. Levels of IL-6 were determined by the B9 murine hybridoma bioassay. Polymerase chain reaction was performed on RNA from control and LPS-treated cells to examine IL-6 message.
      Results: Lipopolysaccharide and PGE2 induced IL-6 release from IEC-6 cells in a dose- and time-dependent fashion, and the substances interacted synergistically. Addition of indomethacin blunted the effect of endotoxin on IL-6 production, consistent with a stimulatory role of PGE2. Polymerase chain reaction demonstrated increased IL-6 messenger RNA in endotoxin-treated cells.
      Conclusions: Endotoxin and PGE2 stimulate IL-6 production in IEC-6 cells and interact synergistically. The endotoxin-stimulated IL-6 release may be regulated at the transcriptional level.
    • Grant Information:
      DK 44201 United States DK NIDDK NIH HHS
    • Accession Number:
      0 (Interleukin-6)
      0 (Lipopolysaccharides)
      0 (RNA, Messenger)
      K7Q1JQR04M (Dinoprostone)
      XXE1CET956 (Indomethacin)
    • Publication Date:
      Date Created: 19941201 Date Completed: 19950104 Latest Revision: 20220317
    • Publication Date:
      20231215
    • Accession Number:
      10.1001/archsurg.1994.01420360080010
    • Accession Number:
      7986158