Evidence against involvement of the acid lysosomal sphingomyelinase in the tumor-necrosis-factor- and interleukin-1-induced sphingomyelin cycle and cell proliferation in human fibroblasts.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Andrieu N;Andrieu N; Salvayre R; Levade T
  • Source:
    The Biochemical journal [Biochem J] 1994 Oct 15; Vol. 303 ( Pt 2), pp. 341-5.
  • Publication Type:
    Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Published by Portland Press on behalf of the Biochemical Society Country of Publication: England NLM ID: 2984726R Publication Model: Print Cited Medium: Print ISSN: 0264-6021 (Print) Linking ISSN: 02646021 NLM ISO Abbreviation: Biochem J Subsets: MEDLINE
    • Publication Information:
      Original Publication: London, UK : Published by Portland Press on behalf of the Biochemical Society
    • Subject Terms:
      Fibroblasts/*drug effects ; Interleukin-1/*pharmacology ; Peptide Fragments/*pharmacology ; Sphingomyelin Phosphodiesterase/*metabolism ; Sphingomyelins/*metabolism ; Tumor Necrosis Factor-alpha/*pharmacology; Cell Division/drug effects ; Cell Line ; Enzyme Activation/drug effects ; Fibroblasts/cytology ; Fibroblasts/metabolism ; Humans ; Hydrolysis/drug effects ; Interleukin-1beta ; Lysosomes/drug effects ; Lysosomes/enzymology ; Mitogens/pharmacology ; Mucolipidoses/enzymology ; Mucolipidoses/genetics ; Mutation/genetics ; Niemann-Pick Diseases/enzymology ; Niemann-Pick Diseases/genetics ; Recombinant Proteins/pharmacology ; Signal Transduction/drug effects ; Signal Transduction/genetics ; Skin/cytology ; Sphingomyelin Phosphodiesterase/deficiency ; Sphingomyelin Phosphodiesterase/drug effects ; Sphingomyelin Phosphodiesterase/genetics
    • Abstract:
      The hydrolysis of sphingomyelin (SPM) has been reported to mediate a number of responses to extracellular agents, including cytokines. The so-called SPM cycle may result from the activation of different types of sphingomyelinases (SPMases). We investigated the hypothetical contribution of acid lysosomal SPMase in the SPM signal-transduction pathway. We examined the ability of human skin fibroblasts with a genetic deficiency of acid lysosomal SPMase activity to respond to tumour necrosis factor alpha (TNF-alpha) or interleukin-1 beta (IL-1 beta). We report that both cytokines promoted SPM hydrolysis in fibroblasts derived from patients with Niemann-Pick disease or I-cell disease, similar to that observed in normal cells. Treatment of normal fibroblasts with cationic amphiphilic drugs resulted in inhibition of acid SPMase activity, but had no effect on cytokine-induced SPM turnover. In addition, TNF-alpha and IL-1 beta stimulated [3H]thymidine incorporation in Niemann-Pick fibroblasts, as in normal cells. Thus our results argue against a role for acid endolysosomal SPMase in mediating the cytokine-induced SPM signalling cascade.
    • References:
      Science. 1992 Mar 27;255(5052):1715-8. (PMID: 1313189)
      Adv Lipid Res. 1993;26:25-48. (PMID: 8379453)
      J Biol Chem. 1994 Feb 25;269(8):5757-63. (PMID: 8119915)
      J Biol Chem. 1991 Jan 5;266(1):484-9. (PMID: 1845977)
      Front Biol. 1979;48:49-130. (PMID: 387466)
      J Biol Chem. 1987 Dec 15;262(35):16759-62. (PMID: 3479432)
      Hoppe Seylers Z Physiol Chem. 1980 May;361(5):755-71. (PMID: 6253377)
      Biochim Biophys Acta. 1993 Dec 21;1154(3-4):223-36. (PMID: 8280742)
      J Biol Chem. 1994 Jan 14;269(2):879-82. (PMID: 7507110)
      Trends Cell Biol. 1992 Aug;2(8):232-6. (PMID: 14731480)
      J Biol Chem. 1993 Sep 25;268(27):20520-3. (PMID: 8376408)
      Adv Prostaglandin Thromboxane Res. 1978;3:1-10. (PMID: 26190)
      J Biol Chem. 1992 Oct 5;267(28):20044-50. (PMID: 1400321)
      Science. 1993 Mar 19;259(5102):1769-71. (PMID: 8456305)
      Anal Biochem. 1985 Oct;150(1):76-85. (PMID: 3843705)
      Anal Biochem. 1977 Nov;83(1):252-7. (PMID: 21598)
      Pharmacol Rev. 1990 Dec;42(4):327-54. (PMID: 2080227)
      Life Sci. 1976 Aug 1;19(3):413-20. (PMID: 822246)
      J Exp Med. 1986 Mar 1;163(3):632-43. (PMID: 3512757)
      J Biol Chem. 1989 Nov 15;264(32):19076-80. (PMID: 2808413)
      Naunyn Schmiedebergs Arch Pharmacol. 1981 Sep;317(2):173-7. (PMID: 7300921)
      FEBS Lett. 1993 Aug 30;329(3):306-12. (PMID: 8365472)
      J Inherit Metab Dis. 1988;11(2):151-7. (PMID: 3139926)
      Lab Invest. 1987 Mar;56(3):234-48. (PMID: 3029503)
      Biochim Biophys Acta. 1990 May 1;1044(1):1-12. (PMID: 2187537)
      Adv Lipid Res. 1993;26:3-23. (PMID: 8379456)
      J Biol Chem. 1994 Feb 4;269(5):3125-8. (PMID: 8106344)
      J Biol Chem. 1994 Feb 11;269(6):4070-7. (PMID: 8307965)
      J Biol Chem. 1994 Feb 18;269(7):5440-5. (PMID: 8106525)
      J Biol Chem. 1983 Jul 25;258(14):8595-600. (PMID: 6863302)
      Science. 1993 Jan 22;259(5094):519-22. (PMID: 8424175)
      FEBS Lett. 1992 Dec 21;314(3):297-300. (PMID: 1334847)
      Biochim Biophys Acta. 1984 Nov 7;777(2):339-42. (PMID: 6091759)
      Biochem J. 1991 Apr 1;275 ( Pt 1):211-7. (PMID: 2018477)
      J Biol Chem. 1989 Apr 5;264(10):5358-63. (PMID: 2538416)
      J Biochem. 1985 Dec;98(6):1669-79. (PMID: 2419314)
      Clin Chim Acta. 1988 Sep 15;176(3):259-67. (PMID: 2846208)
      J Biol Chem. 1957 May;226(1):497-509. (PMID: 13428781)
      Science. 1985 Nov 22;230(4728):943-5. (PMID: 3933111)
      J Biol Chem. 1992 Dec 25;267(36):26121-7. (PMID: 1464623)
      Cell. 1992 Nov 27;71(5):765-76. (PMID: 1330325)
      J Biochem. 1989 Oct;106(4):593-8. (PMID: 2558112)
      J Clin Chem Clin Biochem. 1986 Apr;24(4):205-20. (PMID: 3009683)
    • Accession Number:
      0 (Interleukin-1)
      0 (Interleukin-1beta)
      0 (Mitogens)
      0 (Peptide Fragments)
      0 (Recombinant Proteins)
      0 (Sphingomyelins)
      0 (Tumor Necrosis Factor-alpha)
      106021-96-9 (interleukin-1beta (163-171))
      EC 3.1.4.12 (Sphingomyelin Phosphodiesterase)
    • Publication Date:
      Date Created: 19941015 Date Completed: 19941130 Latest Revision: 20190501
    • Publication Date:
      20240829
    • Accession Number:
      PMC1137332
    • Accession Number:
      10.1042/bj3030341
    • Accession Number:
      7980390