Pulsed field capillary electrophoresis of multikilobase length nucleic acids in dilute methyl cellulose solutions.

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  • Author(s): Kim Y;Kim Y; Morris MD
  • Source:
    Analytical chemistry [Anal Chem] 1994 Oct 01; Vol. 66 (19), pp. 3081-5.
  • Publication Type:
    Journal Article; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print Cited Medium: Print ISSN: 0003-2700 (Print) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Chemical Society.
    • Subject Terms:
      Methylcellulose*; Electrophoresis/*methods ; Nucleic Acids/*isolation & purification; Bacteriophage lambda/genetics ; DNA, Viral/isolation & purification ; Deoxyribonuclease HindIII ; Solutions
    • Abstract:
      Pulsed field capillary gel electrophoresis in dilute methyl cellulose solutions is used to separate nucleic acid fragments in the size range 75-23,000 base pairs. Field inversion is shown to increase resolution for fragments longer than about 500 base pairs. Methyl cellulose solutions as dilute as 0.01% can be used. Intermolecular hydrogen bonding is suggested as the cause of apparent cellulose fiber entanglement at concentrations below the calculated entanglement limit. The 1-kb DNA ladder and the lambda DNA/HindIII restriction fragment mixtures are each baseline resolved in a 28-cm capillary in less than 9 min at 180 V/cm (dc component).
    • Grant Information:
      GM-37006 United States GM NIGMS NIH HHS
    • Accession Number:
      0 (DNA, Viral)
      0 (Nucleic Acids)
      0 (Solutions)
      9004-67-5 (Methylcellulose)
      EC 3.1.21.- (Deoxyribonuclease HindIII)
    • Publication Date:
      Date Created: 19941001 Date Completed: 19941212 Latest Revision: 20190717
    • Publication Date:
      20231215
    • Accession Number:
      10.1021/ac00091a015
    • Accession Number:
      7978303