The intracellular component of cellular 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) reduction is specifically inhibited by beta-amyloid peptides.

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  • Additional Information
    • Source:
      Publisher: Wiley on behalf of the International Society for Neurochemistry Country of Publication: England NLM ID: 2985190R Publication Model: Print Cited Medium: Print ISSN: 0022-3042 (Print) Linking ISSN: 00223042 NLM ISO Abbreviation: J Neurochem Subsets: MEDLINE
    • Publication Information:
      Publication: 2001- : Oxford, UK : Wiley on behalf of the International Society for Neurochemistry
      Original Publication: New York : Raven Press
    • Subject Terms:
      Amyloid beta-Peptides/*pharmacology ; Intracellular Membranes/*metabolism ; Peptide Fragments/*pharmacology ; Tetrazolium Salts/*chemistry ; Thiazoles/*chemistry; Animals ; Cell Membrane Permeability ; Coloring Agents ; HeLa Cells/metabolism ; Humans ; Oxidation-Reduction/drug effects ; PC12 Cells ; Rats ; Tetrazolium Salts/metabolism ; Thiazoles/metabolism
    • Abstract:
      In vitro cell culture model systems for investigating the biochemical mechanisms involved in the neurodegenerative actions of beta-amyloid peptide (beta-AP) have been established. Using rat pheochromocytoma PC12 or human epitheloid HeLa cell lines, submicromolar concentrations of the beta-AP fragments beta 1-40, beta 1-39, and beta 25-35, but not beta 1-28, were found to inhibit the reduction of the redox dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). In both cell lines, the beta-AP-sensitive component represented approximately 70% of total cellular MTT reduction. When the reduction of a series of structurally related dyes was compared with that of MTT, the reduction of 3 alpha-naphthyl-2-phenyl-5-(4-nitrophenyl)-2H-tetrazolium chloride (NTV) was also found to be sensitive to beta 25-35, but that of seven other redox dyes was not. A property common to MTT and NTV is that they are both readily taken up into PC12 and HeLa cells and do not require an artificial electron coupling agent to be reduced. Microscopic analysis of MTT-formazan product formation in PC12 and HeLa cells following beta 25-35 treatment revealed that it was the intracellular component of the reduction of this dye that was abolished. These results support the hypothesis that the cellular reduction of MTT represents a specific indicator of the initial events underlying the mechanism of beta-AP toxicity.
    • Accession Number:
      0 (Amyloid beta-Peptides)
      0 (Coloring Agents)
      0 (Peptide Fragments)
      0 (Tetrazolium Salts)
      0 (Thiazoles)
      EUY85H477I (thiazolyl blue)
    • Publication Date:
      Date Created: 19950701 Date Completed: 19950726 Latest Revision: 20190630
    • Publication Date:
      20221213
    • Accession Number:
      10.1046/j.1471-4159.1995.65010218.x
    • Accession Number:
      7790863