Enhancement of Fibroblast Adhesion and Proliferation on A.oryzae Treated Chitosan Film. (English)

In this study we investigated the effect of A.oryzae treatment of chitosan film on fibroblast adhesion. The number of fibroblast on the A.oryzae treated chitosan film was about 1.8 times higher than that on an untreated chitosan film. The surface of the A.oryzae treated chitosan film was analyzed by X-Ray photoelectron spectroscopy. The A.oryzae treatment introduced nitrogen containing and oxygen containing functional groups onto the surface of the chitosan film. The A.oryzae protein adsorbed on the chitosan film was analyzed by SDS-PAGE and proteome analysis. It was found that three proteins with molecular weights of 104 kDa (beta-glucosidase), 97 kDa (dipeptidyl-peptidase), 92 kDa (dipeptidyl-aminopeptidase) adsorbed on chitosan film, resulting in the increase in the cell adhesion on the film. [ABSTRACT FROM AUTHOR]

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