Microvariation creates significant functional differences in the DR3 molecules.

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  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Country of Publication: United States NLM ID: 8010936 Publication Model: Print Cited Medium: Print ISSN: 0198-8859 (Print) Linking ISSN: 01988859 NLM ISO Abbreviation: Hum Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: [New York] Elsevier/North-Holland.
    • Subject Terms:
      Genetic Variation*; HLA-DR3 Antigen/*genetics; Amino Acid Sequence ; Amino Acids/chemistry ; Animals ; Antibodies, Monoclonal/immunology ; Antigen Presentation ; Cell Line ; DNA, Complementary/genetics ; Fibroblasts ; HLA-DR Antigens/genetics ; HLA-DR3 Antigen/chemistry ; HLA-DR3 Antigen/immunology ; HLA-DRB1 Chains ; Hemagglutinin Glycoproteins, Influenza Virus ; Hemagglutinins, Viral/immunology ; Humans ; Mice ; Models, Molecular ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Peptide Fragments/immunology ; Protein Binding ; Protein Conformation ; Recombinant Proteins/immunology ; Structure-Activity Relationship ; Transfection
    • Abstract:
      Two DR3 molecules differ by four amino acids whose side chains point into the DR antigen-binding groove. To begin to assess the role of microvariation on DR3 function, DRB1*0302 residues were replaced with DRB1*0301 residues at beta-chain positions 26, 47, 86, and 47 plus 86. Murine fibroblast cell lines expressing DR(alpha, beta 1*0301), DR(alpha, beta 1*0302), and the four mutant 0302 molecules were examined for alloproliferative DR(alpha, beta 1*0302)-specific TLC stimulation and peptide binding. Changing position 26 had the most profound effect on T-cell recognition (seven of nine TLCs did not respond). Two TLCs did not respond to the mutant 0302V86 molecule and four TLCs that did respond to this mutant lost responsiveness when positions 47 and 86 were mutated together. These data suggest that each of these variant residues, including position 47, influence T-cell recognition. Surprisingly, none of the mutations had an effect on the absolute binding of HA 307-319 (DR[alpha, beta 1*0302] specific) and HSP 3-13 (DR[alpha, beta 1*0301] specific); however, the mutant 0302 molecules changed at position 86 (glycine to valine) consistently bound HA 307-319 at significantly higher levels than DR(alpha, beta 1*0302). These data for position 86 are in contrast to other DR molecules and indicate that peptide contact residues for a specific DR molecule cannot be predicted based on binding results obtained with other DR molecules. These data suggest that each of these variant groove residues, although not accessible to the TCR, contribute to the significant functional differences between the DR3 microvariants through subtle influences on the DR3-peptide complex.
    • Grant Information:
      P30CA51008 United States CA NCI NIH HHS; R01AI23371 United States AI NIAID NIH HHS
    • Accession Number:
      0 (Amino Acids)
      0 (Antibodies, Monoclonal)
      0 (DNA, Complementary)
      0 (HLA-DR Antigens)
      0 (HLA-DR3 Antigen)
      0 (HLA-DRB1 Chains)
      0 (HLA-DRB1*03:01 antigen)
      0 (Hemagglutinin Glycoproteins, Influenza Virus)
      0 (Hemagglutinins, Viral)
      0 (Peptide Fragments)
      0 (Recombinant Proteins)
    • Publication Date:
      Date Created: 19950101 Date Completed: 19950622 Latest Revision: 20190904
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/0198-8859(94)00074-z
    • Accession Number:
      7751161