Diacylglycerol elevations in control platelets are unaccompanied by pleckstrin phosphorylation. Implications for the role of diacylglycerol in platelet activation.

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  • Author(s): Fukami MH;Fukami MH; Holmsen H
  • Source:
    European journal of biochemistry [Eur J Biochem] 1995 Mar 15; Vol. 228 (3), pp. 579-86.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Country of Publication: England NLM ID: 0107600 Publication Model: Print Cited Medium: Print ISSN: 0014-2956 (Print) Linking ISSN: 00142956 NLM ISO Abbreviation: Eur J Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: -2004: Oxford, UK : Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
      Original Publication: Berlin, New York, Springer.
    • Subject Terms:
      Phosphoproteins* ; Platelet Activation*; Blood Platelets/*metabolism ; Blood Proteins/*metabolism ; Diglycerides/*blood; Blood Platelets/drug effects ; Hot Temperature ; Humans ; In Vitro Techniques ; Kinetics ; Phosphorylation ; Thrombin/pharmacology
    • Abstract:
      Several laboratories have reported that diacylglycerol levels in human platelets (approximately 100 pmol/10(9) platelets) increased severalfold in response to 0.5-1 U/ml thrombin. We report here fluctuations in diacylglycerol mass in control platelets, the magnitude of which were 60-90% of that measured in platelets treated with 0.2-0.5 U/ml of thrombin. These control platelets were not activated by such criteria as absence of aggregation, secretion, phosphatidic acid production and phosphorylation of the protein kinase C substrate, pleckstrin. Thrombin treatment evoked all of the above responses. Analysis of the diacylglycerol molecular species by reverse-phase HPLC of the dimethylated, phosphorylated derivatives showed that all of the molecular species that were present in control platelets were also present in thrombin-treated platelets. Most of the species appeared to fluctuate at random in control platelets with the exception of 1-stearoyl-2-arachidonoyl-sn-glycerol which was more or less stable and increased severalfold over control values only upon thrombin treatment. Furthermore, only this species accumulated as [32P]phosphorylated PtdOH in thrombin-treated platelets prelabelled with [32P]Pi. Our findings show that, in platelets, elevation of diacylglycerol molecular species other than the 1-stearoyl-2-arachidonoyl species occurs, but these changes are not necessarily linked to activation of protein kinase C as measured by pleckstrin phosphorylation which was observed only upon elevation of 1-stearoyl-2-arachidonoyl-sn-glycerol.
    • Accession Number:
      0 (Blood Proteins)
      0 (Diglycerides)
      0 (Phosphoproteins)
      0 (platelet protein P47)
      EC 3.4.21.5 (Thrombin)
    • Publication Date:
      Date Created: 19950315 Date Completed: 19950602 Latest Revision: 20190620
    • Publication Date:
      20240829
    • Accession Number:
      10.1111/j.1432-1033.1995.tb20297.x
    • Accession Number:
      7737151