Deoxypyrimidine cluster mediates the priming by calf thymus DNA primase subunit.

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  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: England NLM ID: 9306673 Publication Model: Print Cited Medium: Print ISSN: 1039-9712 (Print) Linking ISSN: 10399712 NLM ISO Abbreviation: Biochem Mol Biol Int Subsets: MEDLINE
    • Publication Information:
      Publication: 1999: London ; Philadelphia, PA : Taylor & Francis
      Original Publication: Sydney ; New York : Academic Press, c1993-c1999.
    • Subject Terms:
      DNA Replication*; Pyrimidines/*metabolism ; RNA Nucleotidyltransferases/*metabolism; Animals ; Base Sequence ; Binding Sites ; Cattle ; DNA Primase ; Molecular Sequence Data ; Poly A/metabolism ; Poly C/metabolism ; RNA/biosynthesis ; Templates, Genetic ; Thymus Gland/enzymology
    • Abstract:
      Homogeneously purified DNA polymerase alpha subunit-free primase was used to analyze primer RNA synthesis. On a chemically synthesized 36 mer DNA template, a part of upstream region of human c-myc gene, the primer synthesis started from a doublet of deoxythymidine (TT) in the deoxypyrimidine-rich sequence. The primase in DNA polymerase alpha-primase complex synthesized 21-mer reaction product, while DNA polymerase alpha-free primase gave the similar products, 21- and 22-mer, indicating that the site recognition was carried out by primase itself and DNA polymerase alpha subunit has an auxiliary role on it. Product analysis using DNA fragments carrying base substitutions further revealed that the existence of deoxypyrimidine residues around the starting sites was important for priming frequencies. Competition analysis showed that the priming was strongly competed by poly(dC), and to a much lesser extent by poly(dA). Gel-shift analysis showed that the primase could bind to the DNA template, and this complex formation was also competed by poly(dC), but not by poly(dA). These results indicate that primase subunit interacts with the starting site by binding directly with deoxypyrimidine residues.
    • Accession Number:
      0 (Pyrimidines)
      24937-83-5 (Poly A)
      25191-20-2 (poly(dA))
      25609-92-1 (poly(dC))
      30811-80-4 (Poly C)
      63231-63-0 (RNA)
      EC 2.7.7.- (DNA Primase)
      EC 2.7.7.- (RNA Nucleotidyltransferases)
    • Publication Date:
      Date Created: 19930301 Date Completed: 19930611 Latest Revision: 20061115
    • Publication Date:
      20240829
    • Accession Number:
      7683946