T-cell adhesion induced by proteoglycan-immobilized cytokine MIP-1 beta.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print Cited Medium: Print ISSN: 0028-0836 (Print) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
    • Publication Information:
      Publication: Basingstoke : Nature Publishing Group
      Original Publication: London, Macmillan Journals ltd.
    • Subject Terms:
      Cell Adhesion*; Cytokines/*physiology ; Monokines/*physiology ; Proteoglycans/*metabolism ; T-Lymphocytes/*physiology; Cell Adhesion Molecules/metabolism ; Chemokine CCL4 ; Chemotaxis ; Heparin ; Humans ; Macrophage Inflammatory Proteins ; Receptors, Lymphocyte Homing/metabolism ; Serum Albumin, Bovine ; Vascular Cell Adhesion Molecule-1
    • Abstract:
      Lymphocyte migration from blood into tissue depends on integrin-mediated adhesion to endothelium. Adhesion requires not only integrin ligands on the endothelium, but also activation signals because T-cell integrins cannot bind well until they are activated. The physiological 'triggers' for T-cell adhesion are unknown, but cytokines may be good candidates as they are released during inflammation and trigger adhesion in neutrophils and monocytes. We have identified a cytokine, macrophage inflammatory protein-1 beta (MIP-1 beta), that induces both chemotaxis and adhesion of T cells; MIP-1 beta is most effective at augmenting adhesion of CD8+ T cells to the vascular cell adhesion molecule VCAM-1. We reasoned that, as cytokines in vivo will be rapidly washed away, MIP-1 beta might be bound to endothelial surfaces and so induce adhesion in its immobilized form. Here we show that: (1) MIP-1 beta is present on lymph node endothelium; (2) immobilized MIP-1 beta induces binding of T cells to VCAM-1 in vitro. MIP-1 beta was immobilized by binding to proteoglycan: a conjugate of heparin with bovine serum albumin and cellular proteoglycan CD44 were both effective. We propose that MIP-1 beta and other cytokines with glycosaminoglycan-binding sites will bind to and be presented by endothelial proteoglycans to trigger adhesion selectively not only of lymphocyte subsets, but also of other cell types.
    • Comments:
      Comment in: Nature. 1993 Jan 7;361(6407):15-6. (PMID: 8421488)
    • Accession Number:
      0 (Cell Adhesion Molecules)
      0 (Chemokine CCL4)
      0 (Cytokines)
      0 (Macrophage Inflammatory Proteins)
      0 (Monokines)
      0 (Proteoglycans)
      0 (Receptors, Lymphocyte Homing)
      0 (Vascular Cell Adhesion Molecule-1)
      27432CM55Q (Serum Albumin, Bovine)
      9005-49-6 (Heparin)
    • Publication Date:
      Date Created: 19930107 Date Completed: 19930217 Latest Revision: 20220318
    • Publication Date:
      20231215
    • Accession Number:
      10.1038/361079a0
    • Accession Number:
      7678446