DNA fingerprinting of Streptococcus pyogenes from patients with recurrent pharyngotonsillitis by means of random amplified Polymorphic DNA analysis.

Publisher: Informa Healthcare Country of Publication: England NLM ID: 0215333 Publication Model: Print Cited Medium: Print ISSN: 0036-5548 (Print) Linking ISSN: 00365548 NLM ISO Abbreviation: Scand J Infect Dis Subsets: MEDLINE

Publication: 2007-2014 : London : Informa Healthcare
Original Publication: Stockholm, Society for the Publication of Acta medica Scandinavica.

Polymorphism, Genetic*; DNA, Bacterial/*analysis ; Pharyngitis/*microbiology ; Streptococcal Infections/*microbiology ; Streptococcus pyogenes/*genetics ; Tonsillitis/*microbiology; Bacterial Typing Techniques ; Base Sequence ; DNA Fingerprinting/methods ; DNA Primers/chemistry ; Electrophoresis, Agar Gel ; Humans ; Molecular Sequence Data ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Recurrence ; Streptococcus pyogenes/classification ; Streptococcus pyogenes/isolation & purification

In recent years, several DNA fingerprinting methods have been tried for the epidemiological investigation of Streptococcus pyogenes isolates. We chose the Random Amplified Polymorphic DNA (RAPD) method for the typing of pre- and post-antibiotic treatment S. pyogenes isolates obtained from 14 patients with pharyngotonsillitis. Isolates from 4 patients had been collected 10 years earlier than from the others. Hence epidemiological connections between the strains were unlikely. Both pre- and post-antibiotic treatment isolates showed identical RAPD patterns, indicating that recurrences in these patients had been caused by homologous strains of S. pyogenes. It was possible by means of RAPD to differentiate between 12 different T-types and occasionally between clones of the same serotype of S. pyogenes. The RAPD method is an easier alternative to traditional DNA-typing techniques and provides quick results with minimal technical complexity.

0 (DNA Primers)
0 (DNA, Bacterial)

Date Created: 19950101 Date Completed: 19951003 Latest Revision: 20190825

20231215

10.3109/00365549509018990

7660073