Cloning of the 11 beta HSD type II enzyme from human kidney.

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  • Additional Information
    • Source:
      Publisher: Informa Healthcare Country of Publication: England NLM ID: 8408548 Publication Model: Print Cited Medium: Print ISSN: 0743-5800 (Print) Linking ISSN: 07435800 NLM ISO Abbreviation: Endocr Res Subsets: MEDLINE
    • Publication Information:
      Publication: London : Informa Healthcare
      Original Publication: New York, N.Y. : Dekker, c1984-
    • Subject Terms:
      Hydroxysteroid Dehydrogenases/*genetics ; Isoenzymes/*genetics ; Kidney/*enzymology; 11-beta-Hydroxysteroid Dehydrogenases ; Animals ; Cell Line ; Cloning, Molecular ; Corticosterone/metabolism ; Dexamethasone/metabolism ; Female ; Genomic Library ; Humans ; Hydrocortisone/metabolism
    • Abstract:
      The enzyme 11 beta-hydroxysteroid dehydrogenase (11 beta HSD) converts glucocorticoids to receptor inactive metabolites. Two isoforms of the enzyme exist. 11 beta HSD1 is a low affinity NADP dependent enzyme, while 11 beta HSD2 is a high affinity NAD dependent species thought to be responsible for endowing specificity on the mineralocorticoid receptor and for protecting the fetus from high circulating levels of maternal glucocorticoids. We have recently cloned the human renal 11 beta HSD2 enzyme. In this report we show that 11 beta HSD2 potently inactivates the synthetic glucocorticoid dexamethasone, producing a single product thought to be the 11-dehydrodexamethasone metabolite. Sequence analysis shows that the new isoform is a member of the short-chain alcohol dehydrogenase superfamily (SCAD), most closely related to 17 beta HSD2 and distantly related to 11 beta HSD1.
    • Accession Number:
      0 (Isoenzymes)
      7S5I7G3JQL (Dexamethasone)
      EC 1.1.- (Hydroxysteroid Dehydrogenases)
      EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
      W980KJ009P (Corticosterone)
      WI4X0X7BPJ (Hydrocortisone)
    • Publication Date:
      Date Created: 19950201 Date Completed: 19951214 Latest Revision: 20191023
    • Publication Date:
      20221213
    • Accession Number:
      10.3109/07435809509030456
    • Accession Number:
      7588404